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MABF957 Anti-CLEC-2 Antibody, clone AYP1

This mouse monoclonal Anti-CLEC-2 Antibody, clone AYP1, Cat. No. MABF957 is validated for use in Flow Cytometry, Function Assay, and Immunoprecipitation for the detection of CLEC-2.

Anti-CLEC-2 Antibody, clone AYP1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: CLEC1B, CLEC2, UNQ721/PRO1384 UniProt Number: Q9P126
MABF957
100 µL  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF957
DescriptionAnti-CLEC-2 Antibody, clone AYP1
Alternate Names
  • C-type lectin domain family 1 member B
  • C-type lectin-like receptor 2
  • CLEC-2
Background InformationC-type lectin domain family 1 member B (UniProt Q9P126; also known as C-type lectin-like receptor 2, CLEC-2) is encoded by the CLEC1B (also known as CLEC2, UNQ721/PRO1384) gene (Gene ID 51266) in human. CLEC-2 is a type II single-transmembrane (a.a. 34-54) protein with a large extracellular (a.a. 55-229) region that contains a C-type lectin domain (a.a. 109-217) and a short N-terminal cytoplasmic tail (a.a. 1-33) with a single hem-immunoreceptor tyrosine-based activation motif (hemITAM; a.a. 7-10). CLEC-2 expression is restricted to platelets, where it mediates platelets activation via its hemITAM motif, leading to proteolytic cleavage of two other platelet ITAM receptors, glycoprotein (GP)VI and Fc RIIa, but not of CLEC-2 itself. CLEC-2 functions as the receptor for the type I transmembrane GP podoplanin that is widely expressed outside of the vasculature, including lymphatic endothelial cells, type 1 lung alveolar cells, lymph node stromal cells, the choroid plexus epithelium, inflammatory macrophages, as well as a subset of activated T-helper (Th)17 cells. Patients with rheumatoid arthritis show increased plasma levels of CLEC-2-positive microparticles. These microparticles are derived from activated platelets and are negative for GPVI, while microparticles in healthy donors are predominantly derived from megakaryocytes and are positive for both CLEC-2 and GPVI. Platelet-specific deletion of CLEC-2 or one of its downstream signaling proteins, Syk, SLP-76, or PLC 2, leads to a number of developmental problems in mice, including blood-lymphatic mixing in midgestation.
References
Product Information
FormatPurified
PresentationPurified mouse IgG1 in PBS without preservatives.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal Anti-CLEC-2 Antibody, clone AYP1, Cat. No. MABF957 is validated for use in Flow Cytometry, Function Assay, and Immunoprecipitation for the detection of CLEC-2.
Key Applications
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: A representative lot detected doxycycline-dependent expression induction of exogenously transfected CLEC-2/CLEC1B using T-REx 293 cells (Gitz, E., et al. (2014). Blood. 124(14):2262-2270).

Flow Cytometry Analysis: A representative lot (pre-conjugated with Alexa Fluor® 488) detected CLEC-2/CLEC1B expression on the surface of platelets and CD41-positive microparticles in platelet-rich plasma (PRP), but not on monocytes, neutrophils, dendritic cells, B- or T-cells (Gitz, E., et al. (2014). Blood. 124(14):2262-2270).

Function Assay: Clone AYP1 Fab fragment (2.5 µg/mL) cross-linked with anti-mouse Fab-specific F(ab)2 fragments, but not AYP1 Fab or anti-mouse F(ab)2 alone, induced surface P-selectin expression and the aggregation of washed human platelets (Gitz, E., et al. (2014). Blood. 124(14):2262-2270).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CLEC-2/CLEC1B from human platelet lysates. Rhodocytin stimulation prior to cell lysis and IP induced CLEC-2/CLEC1B tyrosine phosphorylation (Gitz, E., et al. (2014). Blood. 124(14):2262-2270).
Biological Information
ImmunogenHis-tagged recombinant human CLEC-2 extracellular fragment.
Epitopeextracellular domain
CloneAYP1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone AYP1 targets an extracellular epitope present in both spliced isoforms of human CLEC-2/CLEC1B reported by UniProt (Q9P126).
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CLEC1B
  • CLEC2
  • UNQ721/PRO1384
Purification MethodProtein G purified.
UniProt Number
Molecular Weight26.60 kDa (isoform 1) and 23.10 kDa (isoform 2). ~32/40 kDa doublet reported due to differential glycosylation (Gitz, E., et al. (2014). Blood. 124(14):2262-2270).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in human platelets.

Flow Cytometry Analysis: 0.2 µL of this antibody detected CLEC-2/CLEC1B on the surface of human platelets.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABF957 04054839055591