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MABT1562-25UL Anti-CFAP43 Antibody, clone 27D5

Anti-CFAP43, clone 27D5, Cat. No. MABT1562, is a mouse monoclonal antibody that detects CFAP43 and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

MABT1562-25UL
25 µL  
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Description
Catalogue NumberMABT1562-25UL
DescriptionAnti-CFAP43 Antibody, clone 27D5
Alternate Names
  • Cilia- and flagella-associated protein 43
Background InformationCilia- and flagella-associated protein 43 (UniProt: E9Q7R9; also known as CFAP43) is encoded by the Cfap43 gene (Gene ID: 100048534) in murine species. CFAP43, a flagellar protein with six WD repeats, is expressed in testis, lung, brain, oviduct, and nasal cavity. It is detected very early during embryonic development It is involved in the regulation of the beating frequency of motile cilia on the epithelial cells. It is also involved in the formation of flagellar axoneme organization and function during spermatogenesis. Mutations in Cfap43 gene have been linked to male infertility. Any malfunction of motile cilia, resulting from defective CFAP43, cause a variety of developmental defects in humans and animal models. These defects may also include impaired mucociliary clearance of airways and hydrocephalus. Cfap43 knockout mice (-/-) exhibit dilation of the third and lateral ventricles. (Ref.: Rachev, E. (2020). Dev. Biol. 459(2); 109-125; Morimoto, Y., et al. (2019). Neurology. 92(20); e2364-e2374; Coutton, C., et al. (2018). Nature Commun. 9; Article 686).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ200
Applications
ApplicationAnti-CFAP43, clone 27D5, Cat. No. MABT1562, is a mouse monoclonal antibody that detects CFAP43 and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunofluorescence
Application NotesTested applications

Western Blotting Analysis: A representative lot detected CFAP43 in Western Blotting applications (Rachev, E., et al. (2020). Dev Biol. 459(2):109-125).

Immunofluorescence Analysis: A representative lot detected CFAP43 in Immunofluorescence applications (Rachev, E., et al. (2020). Dev Biol. 459(2):109-125).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CFAP43 in Immunoprecipitation applications (Rachev, E., et al. (2020). Dev Biol. 459(2):109-125).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenMBP-tagged recombinant fragment corresponding to 174 amino acids from the C-terminal half of mouse Cilia- and flagella-associated protein 43 (CFAP43).
EpitopeC-terminal half
Clone27D5
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 27D5 is a mouse monoclonal antibody that detects Cilia- and flagella-associated protein 43. It targets an epitope within the C-terminal half.
IsotypeIgG2b
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Cfap43
Purification MethodProtein G purified
UniProt Number
Molecular Weight~190 kDa observed; 193.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Mouse testis tissue extracts.

Western Blotting Analysis: A 1:500 dilution of this antibody detected CFAP43 in Mouse testis tissue extracts.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABT1562-25UL 04065269135104