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CBL1366 Anti-CD140a Antibody, clone APA5

CBL1366
100 µg  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Speciální nabídky

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
MELISA, FC, WB, IHC, FUNCRPurifiedMonoclonal Antibody
Description
Catalogue NumberCBL1366
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-CD140a Antibody, clone APA5
Alternate Names
  • PDGF Receptor alpha
Product Information
FormatPurified
PresentationPurified immunoglobulin in PBS, 150 mM NaCl, pH 7.2, containing 0.09% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect CD140a using this Anti-CD140a Antibody, clone APA5 validated for use in ELISA, FC, WB, IH.
Key Applications
  • ELISA
  • Flow Cytometry
  • Western Blotting
  • Immunohistochemistry
  • Affects Function
Application NotesFlow Cytometry: <= 1 μg per 10E6 cells

ELISA

Immunohistochemistry: 10μg/mL

Blocking of Ligand Binding

Western Blotting

Optimal working dilutions must be determined by the end user.
Biological Information
CloneAPA5
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRat
SpecificitySpecifically recognizes mouse CD140a, the alpha chain of PDGF receptor. A receptor tyrosine kinase, PDGFRa forms dimers on the surface upon ligand binding and phosphorylates substrates. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
Gene Symbol
  • PDGFRA
  • CD140a
  • PDGFR2
  • Rhe-PDGFRA
  • PDGF-R-alpha
  • CD140A
  • MGC74795
  • EC 2.7.10.1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P16234 # Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
SIZE: 1089 amino acids; 122670 Da
SUBUNIT: Homodimer, and heterodimer with PDGFRB. Interacts with SHB (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P16234 # A fusion of PDGFRA and FIP1L1 (FIP1L1-PDGFRA), due to an interstitial chromosomal deletion, is the cause of some cases of hypereosinophilic syndrome (HES) [MIM:607685]. HES is a rare hematologic disorder characterized by sustained overproduction of eosinophils in the bone marrow, eosinophilia, tissue infiltration and organ damage.
SIMILARITY: SwissProt: P16234 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C for up to 12 months from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Katalogové číslo GTIN
CBL1366 04053252322754