Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABF2791-100UG Anti-CCR9 Antibody, clone 7E7

Anti-CCR9, clone 7E7, Cat. No. MABF2791, is a rat monoclonal antibody that detects CCR9 and is tested for use in Flow Cytometry, Immunofluorescence, and Immunohistochemistry.

MABF2791-100UG
100 μg  
Purchase on Sigma-Aldrich

Přehled

Replacement Information
Description
Catalogue NumberMABF2791-100UG
DescriptionAnti-CCR9 Antibody, clone 7E7
Alternate Names
  • C-C chemokine receptor type 9
  • C-C CKR-9
  • CC-CKR-9
  • CCR-9
  • Chemokine C-C receptor 10
  • CDw199
Background InformationC-C chemokine receptor type 9 (UniProt: Q9WUT7; also known as C-C CKR-9, CC-CKR-9, CCR-9, Chemokine C-C receptor 10, CDw199) is encoded by the Crr9 (also known as Cmkbr10) gene (Gene ID: 12769) in murine species. CCR9 is a multi-pass membrane protein of the G-protein coupled receptor 1 family. It has four extracellular domains, seven transmembrane domains, and four cytoplasmic domains and serves as a receptor for chemokine CCL25/TECK. It is highly expressed in the thymus tissue. It is also expressed on virtually all small intestinal T cells and IgA-producing plasma cells (PCs) from the spleen. It is reported to be crucial for the positioning of PCs to the small intestine. Higher expression of CCR9 is observed in newly generated PCs and it is down-regulated once these cells reach their final destination in the intestine. In wild-type mice, a majority of the PCs are shown to locate to the lower half of the villus where CCL25 is also expressed at high levels. CCR9 deficient mice exhibit reduced number of PCs in the intestine and are unable to mount a T cell dependent antigen specific IgA response. CCR9-/- hematopoietic stem cells are deficient in their ability to generate all major thymocyte subsets. (Ref.: Svensson, M., et al. (2008). J. Leukoc. Biol. 83(1); 156-164; Pabst, O., et al. (2004). J. Exp. Med. 199(3); 411-416).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-CCR9, clone 7E7, Cat. No. MABF2791, is a rat monoclonal antibody that detects CCR9 and is tested for use in Flow Cytometry, Immunofluorescence, and Immunohistochemistry.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
  • Immunofluorescence
Application NotesTested Applications

Immunohistochemistry Applications: A representative lot detected CCR9 in Immunohistochemistry applications (Guo, Z., et al. (2008). Int Immunol. 20(3):307-15; Pabst, O., et al. (2004). J Exp Med. 199(3):411-6; McGuire, H.M., et al. (2011). Immunity. 34(4):602-15).

Flow Cytometry Analysis: A representative lot detected CCR9 in Flow Cytometry applications (Guo, Z., et al. (2008). Int Immunol. 20(3):307-15; Pabst, O., et al. (2004). J Exp Med. 199(3):411-6; McGuire, H.M., et al. (2011). Immunity. 34(4):602-15; Svensson, M., et al. (2008). J Leukoc Biol. 83(1):156-64; Chennupati, V., et al. (2010). J Immunol. 185(9):5160-8).

Immunofluorescence Analysis: A representative lot detected CCR9 in Immunofluorescence applications (Guo, Z., et al. (2008). Int Immunol. 20(3):307-15; Pabst, O., et al. (2004). J Exp Med. 199(3):411-6; McGuire, H.M., et al. (2011). Immunity. 34(4):602-15).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 20 amino acids from the first extracellular domain in the N-terminal region of mouse C-C-chemokine receptor type 9 (CCR9).
EpitopeN-terminal
Clone7E7
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 7E7 is a rat monoclonal antibody that detects C-C chemokine receptor type 9 (CCR9). It targets an epitope within 20 amino acids from the fist extracellular domain in the N-terminal region.
IsotypeIgG2b
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Ccr9
Purification MethodProtein G purified
UniProt Number
Molecular Weight41.91 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in BALB/c mouse thymocytes.

Flow Cytometry Analysis: 1 µg of this antibody detected CCR9 in one million BALB/c mouse thymocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABF2791-100UG 04065268363294