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ABT1373 Anti-Aggrecan

Anti-Aggrecan, Cat. No. ABT1373, is a rabbit polyclonal antibody that detects Aggrecan core protein and is tested for use in Immunohsitochemistry, Immunohistochemistry (Paraffin), Radioimmunoassay, and Western Blotting.

Anti-Aggrecan MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Acan, Agc, Agc1 UniProt Number: P07897
ABT1373
100 µL  
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Replacement Information

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
RWB, IHC, RIA, IH(P)RbSerumPolyclonal Antibody
Description
Catalogue NumberABT1373
DescriptionAnti-Aggrecan
Alternate Names
  • Aggrecan core protein
  • Cartilage-specific proteoglycan core protein
  • CSPCP
Background InformationAggrecan core protein (UniProt: P07897; also known as Cartilage-specific proteoglycan core protein, CSPCP) is encoded by the Acan (also known as Agc, Agc1) gene (GeneID: 58968) in rat. Aggrecan is a secreted proteoglycan that is a major component of extracellular matrix of cartilagenous tissues. Its major function is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region and may play a regulatory role in the matrix assembly of the cartilage. Aggrecan contains two globular domains, G1 and G2 that comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. The G1 region is located in the N-terminus of the core protein and interacts with hyaluronic acid (HA). G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. The G2 region is composed of two B-like domains, but does not have the ability to interact with HA. G2 region is separated from the G1 region by the interglobular domain, which is a prominent site for proteolysis. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3. Aggrecan is present in the extracellular matrix in the form of proteoglycan aggregates, in which many aggrecan molecules interact with hyaluronan and a link protein stabilizes each interaction. It is reported that aggrecan structure changes throughout the life cycle due to changes in synthetic and degradative events. Changes due to synthesis alter the structure of the chondroitin sulfate and keratan sulfate chains, whereas those due to degradation cause cleavage of all components of the aggregate. Aggrecan is also present in most of the retina including the outer nuclear layer, the outer plexiform layer, and the inner plexiform layer.
References
Product Information
FormatSerum
HS Code3002 15 90
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Aggrecan, Cat. No. ABT1373, is a rabbit polyclonal antibody that detects Aggrecan core protein and is tested for use in Immunohsitochemistry, Immunohistochemistry (Paraffin), Radioimmunoassay, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Radioimmunoassay
  • Immunohistochemistry (Paraffin)
Application NotesRadioimmunoassay Analysis: A representative lot detected Aggrecan in Postnatal rat brain (Milev, P., et. al. (1998). Biochem Biopys Res Commun. 247(2):207-12).

Immunohistochemistry Analysis: A representative lot detected Aggrecan in Embryonic day 16 rat eye, P0, P9 and adult rat retina (Popp, S., et. al. (2004). Exp Eye Res. 79(3):351-6) and Embryonic day 16, 19 rat brain and postnatal day 7 cerebellum, embryonic day 19 spinal cord (Popp, S., et. al. (2003). Dev Dyn. 227(1):143-9).

Western Blotting Analysis: A representative lot detected Aggrecan in purified proteoglycans from a PBS extract of 7-day rat brain with chondroitinase ABC treatment (Popp, S., et. al. (2003). Dev Dyn. 227(1):143-9).
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 213 amino acids from the C-terminal half of rat Aggrecan.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects aggrecan in rat eye sections.
IsotypeIgG
Species Reactivity
  • Rat
Species Reactivity NoteRat.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Acan
  • Agc
  • Agc1
Purification MethodUnpurified
UniProt Number
Molecular Weight221.12 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in rat retina tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Aggrecan in rat retina tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABT1373 04054839240546