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MABE881 Anti-26S proteasome regulatory subunit RPN11, clone 7C7.1 Antibody

This Anti-26S proteasome regulatory subunit RPN11, clone 7C7.1 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of 26S proteasome regulatory subunit RPN11.

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Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology, Cell Cycle, DNA Replication & Repair Gene Symbol: PSMD14, POH1 UniProt Number: O00487
MABE881
100 µg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE881
DescriptionAnti-26S proteasome regulatory subunit RPN11, clone 7C7.1 Antibody
Alternate Names
  • 26S proteasome regulatory subunit RPN11
  • 26S proteasome-associated PAD1 homolog 1
  • 26S proteasome non-ATPase regulatory subunit 14
Background Information26S proteasome non-ATPase regulatory subunit 14 is also known as 26S proteasome regulatory subunit RPN11 or 26S proteasome-associated PAD1 homolog 1. This protein is expressed from the PSMD14 gene and is the metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. 26S proteasome non-ATPase regulatory subunit 14 has a role in the response to double-strand breaks (DSBs) by regulating non-homologous end joining (NHEJ) via cleaving 'Lys-63'-linked polyubiquitin, retaining JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. 26S proteasome non-ATPase regulatory subunit 14 is also involved in homologous recombination repair by promoting RAD51 loading. 26S proteasome non-ATPase regulatory subunit 14 is widely expressed, but found in the highest levels in heart and skeletal muscle.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-26S proteasome regulatory subunit RPN11, clone 7C7.1 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of 26S proteasome regulatory subunit RPN11.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected 26S proteasome regulatory subunit RPN11 in human cardiac myocytes and rat skelatal muscle myocytes.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 26S proteasome regulatory subunit RPN11.
EpitopeRegion near N-terminus
Clone7C7.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityRecognizes 26S proteasome regulatory subunit RPN11
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PSMD14
  • POH1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~ 35 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HT-1080 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected 26S proteasome regulatory subunit RPN1 in 10 µg of HT-1080 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABE881 04053252986529