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MABE302 Anti-2,2,7-Trimethylguanosine Antibody, clone K121

Anti-2, 2, 7-Trimethylguanosine Antibody, clone K121 is a high quality Mouse Monoclonal Antibody for the detection of 2, 2, 7-Trimethylguanosine & has been validated in ICC, PAI & IP.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins
MABE302
100 µg  
Purchase on Sigma-Aldrich

Speciální nabídky

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Replacement Information

Speciální nabídky

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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MICC, IAP, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE302
DescriptionAnti-2,2,7-Trimethylguanosine Antibody, clone K121
Background InformationThe 2,2,7-trimethylguanosine cap structure is found on the 5’ end of small nuclear RNAs and small nucleolar RNAs. Initially, these RNA molecules are capped with 7-monomethylguanosine (m7G) which is then methylated by the trimethylguanosine synthase enzyme to form 2,2,7-trimethylguanosine. The synthesis of 2,2,7-trimethylguanosine occurs in the cytoplasm and is an important marker for nuclear localization of RNA molecules. Capping of RNAs is an important requirement for RNA transport and splicing processes. Uncapped RNAs are rapidly degraded by the 5′ exoribonuclease enzyme. 2,2,7-trimethylguanosine capping may play a role in the expression of viral RNAs.
References
Product Information
FormatPurified
Control
  • NIH/3T3, HeLa, and A431 cells
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-2, 2, 7-Trimethylguanosine Antibody, clone K121 is a high quality Mouse Monoclonal Antibody for the detection of 2, 2, 7-Trimethylguanosine & has been validated in ICC, PAI & IP.
Key Applications
  • Immunocytochemistry
  • Immunoaffinity Purification
  • Immunoprecipitation
Application NotesImmunoaffinity Purification Analysis: A representative lot from an independent laboratory was used in IAP (Krainer, A.R., et al. (1988). Nucleic Acids Res. 16(20):9415-9429.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory was immunoprecipitated in IP (Moketi, S., et al. (2002). Mol Cell. 10(3):599-609.).
Biological Information
Immunogen2,2,7-trimethylguanosine
CloneK121
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Purification MethodProtein G Purified
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in NIH/3T3, HeLa, and A431 cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected 2,2,7-trimethylguanosine in NIH/3T3, HeLa, and A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABE302 04053252595974

Documentation

Anti-2,2,7-Trimethylguanosine Antibody, clone K121 MSDS

Title

Safety Data Sheet (SDS) 

Anti-2,2,7-Trimethylguanosine Antibody, clone K121 Certificates of Analysis

TitleLot Number
Anti-2,2,7-Trimethylguanosine, clone -2733258 2733258
Anti-2,2,7-Trimethylguanosine, clone -2792459 2792459
Anti-2,2,7-Trimethylguanosine, clone -2794502 2794502
Anti-2,2,7-Trimethylguanosine, clone K121 - 2469297 2469297
Anti-2,2,7-Trimethylguanosine, clone K121 - 2227177 2227177
Anti-2,2,7-Trimethylguanosine, clone K121 - 3394664 3394664
Anti-2,2,7-Trimethylguanosine, clone K121 - 3585811 3585811
Anti-2,2,7-Trimethylguanosine, clone K121 - 3698177 3698177
Anti-2,2,7-Trimethylguanosine, clone K121 - 3729383 3729383
Anti-2,2,7-Trimethylguanosine, clone K121 - 3819932 3819932
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References

Reference overviewPub Med ID
Pre-mRNA splicing by complementation with purified human U1, U2, U4/U6 and U5 snRNPs.
Krainer, A R
Nucleic Acids Res., 16: 9415-29 (1988)  1987

Zobrazit abstrakt 		3141901 3141901

Brochure

Title
New Products: Volume 3, 2012