Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

UFC910024 Amicon® Ultra-15 Centrifugal Filter Unit

Ultracel-100 regenerated cellulose membrane, 15 mL sample volume

Amicon® Ultra-15 Centrifugal Filter Unit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Protein Extraction, Protein Purification
UFC910024
24  
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Key ApplicationsNMWLVolumeMinimum Final Concentrate Volume
PExt, Protein Purification100 KDa15 mL200 µL
Description
Catalogue NumberUFC910024
Trade Name
  • Amicon Ultra
DescriptionAmicon® Ultra-15 Centrifugal Filter Unit
Background InformationAMICON ULTRA-15 15ML - 100 KDa cutoff. Available in 3 pack sizes.
UFC910008 - 8 pack
UFC910024 - 24 pack
UFC910096 - 96 pack



High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
High retentate recovery of greater than 90%
Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
Heat-sealed membrane minimizes downstream extractables
100% integrity tested for reliable performance
Convenient sample monitoring with translucent housing and volume gradations
Direct pipettor sample access eliminates processing step to recover concentrate
High concentration factors of 80–100X

Applications:
Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS
Concentration of dilute or pre-purified proteins from column eluents
Desalting, buffer exchange and protein dialysis
References
Product Information
Filter CodePLHK
Color CodeClear with pink cap
Filter TypeUltrafiltration
NMWL100 KDa
Quality LevelMQ400
Applications
ApplicationUltracel-100 regenerated cellulose membrane, 15 mL sample volume
Key Applications
  • Protein Extraction
  • Protein Purification
Biological Information
SterilityNon-Sterile
Physicochemical Information
Centrifugal ApplicationProtein Concentration and Desalting
Dimensions
Length12.1 cm (4.807 in.)
Diameter2.97 cm
Filtration Area7.6 cm²
Minimum Final Concentrate Volume200 µL
Volume15 mL
Materials Information
Chemistry
  • Regenerated Cellulose (RC)
Device Material
  • Housing: Styrene/butadiene
  • Tube: Polypropylene
  • Cap and liner: Polyethylene
Support MaterialRegenerated cellulose
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Packaging Information
Material Size24
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
UFC910024 04053252317439

Documentation

References

Reference overviewPub Med ID
Specific Lipids Modulate the Transporter Associated with Antigen Processing (TAP).
Schölz, Christian, et al.
J. Biol. Chem., 286: 13346-56 (2011)  2010

Zobrazit abstrakt 		21357424 21357424

Certificates of Quality

Title
UFC910024 - 0000241948

Brochure

Title
REFINE PROTEIN PREPARATION. Tools for better protein analysis

FAQ

QuestionAnswer
Amicon Ultra units are offered in what volumes?Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes.
What is the recommended rotor for Amicon Ultra units?Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units.
How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra?Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest.
Which membranes are available in Amicon Ultra units?Amicon Ultra units are only available with Ultracel regenerated cellulose material. This high recovery/low binding membrane is offered in 3,000, 10,000, 30,000, 50,000 & 100,000 MWCO.
Can the Amicon Ultra be spun to dryness?No, the Amicon Ultra has a built in deadstop.
Can the Amicon Ultra be used with nucleic acids?Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide.
What is the maximum sample volume for Amicon Ultra?
Amicon Ultra-4Amicon Ultra-15
Swinging Bucket4ml 15ml
Fixed Angle4ml(35 & 45 degree rotor angle)12ml(35 degree rotor angle)
3.5ml(23 degree rotor angle)
What is the chemical compatibility of Amicon Ultra?Amicon Ultra units are intended for use with biological fluids and aqueous solutions. Please refer to the user guide, catalogue, or contact Technical Support.
Can Amicon Ultra units be sterilized with ethanol?Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization.
Do you have any centrifugal filter units that are pyrogen free?We do not have any centrifugals devices that we claim are pyrogen free. Even the sterile devices could contain pyrogens.
Show more

User Guides

Title
Amicon® Ultra-15 Centrifugal Filter Devices Non IVD User guide