Millipore Sigma Vibrant Logo

70663 Proteinase K, Lyophilized

Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA.

Proteinase K, Lyophilized: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
View Products on Sigmaaldrich.com
70663
Ver precios y disponibilidad

Descripción

Replacement Information

Precios y disponibilidad

Número de referencia DisponiblidadEmbalaje Cant./Env. Precio Cantidad
70663-4
Comprobando disponibilidad...
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

      		 Ampolla de plást. 100 mg
      Recuperando precio...
      No pudo obtenerse el precio
      La cantidad mínima tiene que ser múltiplo de
      Maximum Quantity is
      Al finalizar el pedido Más información
      Ahorró ()
       
      —
      Solicitar precio
      		 —
      70663-5
      Comprobando disponibilidad...
      —
      Disponibilidad a confirmar Disponibilidad a confirmar
      Ingrese cantidad 
      Suspendido
      Cantidades limitadas disponibles
      Debe confirmarse disponibilidad
        El resto: se avisará
          El resto: se avisará
          Se avisará
          Póngase en contacto con el Servicio de Atención al Cliente
          Contact Customer Service

          		 Frasco de vidrio 500 mg
          Recuperando precio...
          No pudo obtenerse el precio
          La cantidad mínima tiene que ser múltiplo de
          Maximum Quantity is
          Al finalizar el pedido Más información
          Ahorró ()
           
          —
          Solicitar precio
          		 —
          Description
          OverviewProteinase K is a highly active 28,904-Da serine protease isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA. Its activity is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C). The recommended working concentration is 50-100 µg/ml for protein removal and enzyme inactivation, and up to 2 mg/ml for tissue treatment. The Proteinase K, Lyophilized powder can be prepared as a 20 mg/ml stock solution in water and stored in aliquots at -20°C. The enzyme is also available as a ready-to-use concentrated stock solution (600 mAU/ml) that is convenient for routine use in most applications. 1 mg of Proteinase K is the equivalent of 30 mAU (AU = Anson unit). The Novagen Proteinase K products are free of detectable DNase and RNase
          Catalogue Number70663
          Brand Family Novagen®
          References
          Product Information
          CAS number39450-01-6
          Unit of DefinitionOne AU (AU = Anson unit) is defined as the amount of enzyme that liberates 1.0 µmol (181 µg) of tyrosine from casein per minute at pH 7.5 at 37°C.
          Quality LevelMQ400
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/37/38-42

          Irritating to eyes, respiratory system and skin.
          May cause sensitization by inhalation.
          S PhraseS: 22-24-26-36/37

          Do not breathe dust.
          Avoid contact with skin.
          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing and gloves.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Harmful
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Número de referencia GTIN
          70663-4 04055977257670
          70663-5 07790788059859

          Documentation

          Proteinase K, Lyophilized Ficha datos de seguridad (MSDS)

          Título

          Ficha técnica de seguridad del material (MSDS) 

          Proteinase K, Lyophilized Certificados de análisis

          CargoNúmero de lote
          70663

          Folleto

          Cargo
          High fidelity gene amplification
          The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

          Citas

          Título
        • Shannon M. Anderson, et al. (2007) New markers for murine memory B cells that define mutated and unmutated subsets. Journal of Experimental Medicine 204, 2103-2114.
        • Andrew A. Horwitz, et al. (2007) ATP-induced structural transitions in PAN, the proteasome-regulatory ATPase complex in Archaea. Journal of Biological Chemistry 282, 22921-22929.
          		•

          Protocolos de usuario

          Cargo
          TB271 Proteinase K

          Productos y aplicaciones relacionados

          Related Products

          Número de referencia Descripción  
          71049 Proteinase K Solution, 600 mAU/ml Precios y disponibilidad

          Categorías

          Life Science Research > Proteins and Enzymes > Other Enzymes
          Life Science Research > Genomic Analysis > DNA Preparation & Cloning > DNA Purification > DNA/RNA Clean-Up