Separation of 80 mg/Injection


Column Chromolith® SemiPrep RP-18 endcapped 100-10 mm Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Diagrams/LE-Chrom-Chromolith SemiPrep-Separation of 80 mg injection-480x161-20140214.png
By courtesy of Dr. A. Espada and C. Anta, Lilly Spain
Mobile phase A: Acetonitrile with 0.05% TFA
B: Water with 0.05% TFA
Gradient 0-1 min
1-5 min
5-5.2 min
5.2-6.2 min
5% A
5 - 90% A
95% A
95% A
Flow rate 8 mL/min
Detection UV 254 nm
Injection volume 400 μL
Sample 1. Propranolol
2. Nifedipine
Dissolved in
DMSO/Methanol 1/1
200 mg/mL
200 mg/mL