Acetonitrile/water 60/40, data for anthracene (3rd peak)


Mobile phase Acetonitrile/
water 60/40
Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Diagrams/LE-Chrom-Chromolith SemiPrep-Acetonitrile water-480x315-20140214.png
Flow rate 2 mL/min
Detection UV 254 nm
Temperature ambient
Injection volume 5 μL
Sample 1. Thiourea
2. Progesterone 3. Anthracene