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69049 Pellet Paint® Co-Precipitant

A fluorescent dye-labeled carrier formulated specifically for use in alcohol precipitation of nucleic acids (DNA and RNA pellets)

Pellet Paint® Co-Precipitant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69049
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69049-3
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      		 Plastic ampoule 125 rxn
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      Description
      OverviewPellet Paint® Co-Precipitant is a visible dye-labeled carrier formulated specifically for use in alcohol precipitation of nucleic acids (McCormick 1995, McCormick 1996). The two-minute precipitation uses just 2 µl per reaction and requires no low-temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink color. The pellet can be easily followed during washing steps and prevents losses during handling.

      Most applications of PCR benefit from a clean-up step in which primers and other reactants are removed and the target DNA is concentrated (Taggart 1998). Pellet Paint Co- Precipitant is ideal for this cleanup because the procedure is rapid, primers < 50 nt in length are efficiently removed, and the DNA is quantitatively recovered. Furthermore, it is easy to tell when the DNA has been completely resuspended following the precipitation step.

      Pellet Paint Co-Precipitant is compatible with most molecular biology procedures and is free of contaminating nucleic acids and nucleolytic enzymes. Although it absorbs in the UV range, accurate spectrophotometric measurements of DNA or RNA samples are possible; the absorbance ratio (provided with each package) can be used as a correction factor when determining nucleic acid concentration (McCormick 1996). Pellet Paint Co-Precipitant is compatible with automatted Cy5® sequencers. Pellet Paint NF Co- Precipitant is recommended for use with PE Applied Biosystems automated sequencers.
      Catalogue Number69049
      Brand Family Novagen®
      Features and benefits• Direct visualization and tracking of precipitated material
      References
      ReferencesMcCormick, M. 1995. inNovations 4a, 10. McCormick, M. 1996. inNovations 5, 10. Taggart, E. W., et al. 1998. J. Clin. Microbiol. 36, 3408.
      Product Information
      •250 µl or 2 mlPellet Paint Co-Precipitant
      •1 ml or 8 ml3 M Sodium Acetate, pH 5.2
      DeclarationThis product is covered by U.S. Patents 7,550,447, 7,144,713, EP Patent 853,680, Canadian Patent 2,231,501 owned by EMD Chemicals Inc. or its Affiliates
      FormLiquid
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      69049-3 04055977259797

      Documentation

      Pellet Paint® Co-Precipitant SDS

      Title

      Safety Data Sheet (SDS) 

      Pellet Paint® Co-Precipitant Certificates of Analysis

      TitleLot Number
      69049

      References

      Reference overview
      McCormick, M. 1995. inNovations 4a, 10. McCormick, M. 1996. inNovations 5, 10. Taggart, E. W., et al. 1998. J. Clin. Microbiol. 36, 3408.

      Brochure

      Title
      High fidelity gene amplification

      Citations

      Title
    • Brooks, C.L., 2007. Journal of Biological Chemistry 282, 22804.
    • Buttner, M.P., et al. 2007. Applied and Enviornmental Microbiology 73, 3505.
    • Halling, R.E., et al. 2007. Mycologia 99, 310.
    • Quan, S., et al. 2007. Journal of Biological Chemistry 282, 28823.
    • Tsai, C.J., et al. 2007. Procedings of the National Academy of Science 104, 7851.
    • Nowak, J.E., et al. 2006. Bio/Techniques 40, 220.
    • Lee, Y., et al. 2005 Clinical Cancer Research 11, 1059.
    • Mori, T., et al. 2005. Journal of Clinical Oncology 23, 9351.
    • Pendleton, L.C., et al. 2005. Journal of Biological Chemistry 280, 24252.
    • Beischlag, T.V., et al. 2004. Journal of Biological Chemistry 279, 54620.
    • Xu, M., et al. 2002. Journal of Clinical Microbiology 40, 2626.
    • Taggart, E.W., et al. 1998. Journal of Clinical Microbiology 36, 3408.
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      User Protocols

      Title
      TB146 Pellet Paint® Co-Precipitant