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109253 Sigma-AldrichPapanicolaou's solution 1a Harris' hematoxylin solution

for cytology

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
User Guides

Synonyms: Hematoxylin solution acc. to Harris, Colour solution acc. to Papanicolaou, Harris' Hematoxylin solution

Recommended Products

Overview

Replacement Information

Pricing & Availability

Catalogue Number	Availability	Packaging	Qty/Pack	Price	Quantity
1092530500	Retrieving availability... — Limited Availability In Stock Discontinued Limited Quantities Available Availability to be confirmed Login to See Inventory In Stock Limited Availability In Stock Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Plastic bottle	500 ml	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites
1092531000	Retrieving availability... — Limited Availability In Stock Discontinued Limited Quantities Available Availability to be confirmed Login to See Inventory In Stock Limited Availability In Stock Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Plastic bottle	1 l	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites
1092531022	Retrieving availability... — Limited Availability In Stock Discontinued Limited Quantities Available Availability to be confirmed Login to See Inventory In Stock Limited Availability In Stock Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Plastic bottle	20 PC	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites
1092532500	Retrieving availability... — Limited Availability In Stock Discontinued Limited Quantities Available Availability to be confirmed Login to See Inventory In Stock Limited Availability In Stock Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Plastic bottle	2.5 l	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites
CO1C1092531000	Retrieving availability... — Limited Availability In Stock Discontinued Limited Quantities Available Availability to be confirmed Login to See Inventory In Stock Limited Availability In Stock Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service		1 l	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites

Description
Catalogue Number	109253
Synonyms	Hematoxylin solution acc. to Harris, Colour solution acc. to Papanicolaou, Harris' Hematoxylin solution
Description	Papanicolaou’s solution 1a Harris hematoxylin solution
Overview	Papanicolaou’s solution 1a Harris hematoxylin solution - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou’s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Hematoxylin solution modified acc.to Gill II, (Cat.no. 105175), Papanicolaou’s solution 1a Harris hematoxylin solution, (Cat.no. 109253) or Papanicolaou’s solution 1b Hematoxylin solution S, (Cat.no. 109254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by (Papanicolaou’s solution 2b Orange II solution, (Cat. no. 1106887) or Papanicolaou’s solution 2a Orange G solution (OG6), (Cat. no. 106888)), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, Papanicolaou’s solution 3a polychromatic solution EA 31, (Cat.no 109271), Papanicolaou’s solution 3b polychromatic solution EA 50, (Cat.no. 109272), Papanicolaou’s solution 3c polychromatic solution EA 65,(Cat.no. 109270), or Papanicolaou’s solution 3d polychromatic solution EA 65, (Cat.no.109269). The polychrome solution is used for demonstration of differentiation of squamous cells. Both Papanicolaou’s solution 1a Harris hematoxylin solution(Cat.no. 109253) and Papanicolaou’s solution 1b Hematoxylin solution S (Cat.no. 109254) give a blue to dark violet staining result with clinical specimen material. A 500 ml bottle of either solution is sufficient for 1500 -2500 stainings. It is registered as IVD and CE certified product and can be used for clinical diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

References

Product Information
HS Code	3212 90 00
Quality Level	MQ400

Applications
Application	for cytology

Biological Information

Physicochemical Information
Boiling point	100 °C (1013 hPa)
Density	1.04 g/cm3 (20 °C)
pH value	2.5 - 3.0 (H₂O, 20 °C)

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS
Hazard Pictogram(s)
Hazard Statement(s)	H318: Causes serious eye damage. EUH210: Safety data sheet available on request.
Precautionary Statement(s)	P280: Wear eye protection/ face protection. P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Signal Word	Danger
Storage class	10 - 13 Other liquids and solids
WGK	WGK 1 slightly hazardous to water
Disposal	28 Aqueous solutions: Container D.

Safety Information

Product Usage Statements

Storage and Shipping Information
Storage	Store at +15°C to +25°C.

Packaging Information

Transport Information

Supplemental Information

Specifications
Suitability for microscopy (Vaginal smear)	passes test
Nuclei	blue to dark violet
Cyanophilic Cytoplasm (basophilic)	blue-green
Eosinophilic Cytoplasm (acidophilic)	pink

Global Trade Item Number
Catalogue Number	GTIN
1092530500	04022536112466
1092531000	04022536676661
1092531022	04022536707433
1092532500	04022536112480
CO1C1092531000	04061839008625

Documentation

Papanicolaou's solution 1a Harris' hematoxylin solution SDS

Title
Safety Data Sheet (SDS)

Papanicolaou's solution 1a Harris' hematoxylin solution Certificates of Analysis

Product Number	Packaging	Specification	Lot Number
1092530500	Plastic bottle	PDF Specification Document	Enter Lot Number
1092531000	Plastic bottle	PDF Specification Document	Enter Lot Number
1092531022	Plastic bottle	PDF Specification Document	Enter Lot Number
1092532500	Plastic bottle	PDF Specification Document	Enter Lot Number
CO1C1092531000		PDF Specification Document	Enter Lot Number

User Guides

Title
109253 Solución de Papanicolaou 1a solución de hematoxilina según Harris para citología
109253 Solución de Papanicolaou 1a solución de hematoxilina según Harris para citología (versión de Cuba)
User Guide (IFU) - 109253

Accessories (Primary)

Catalogue Number

Description

106887

Papanicolaou’s solution 2b Orange II solution

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106888

Papanicolaou's solution 2a Orange G solution (OG 6)

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109254

Papanicolaou's solution 1b Hematoxylin solution S

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109271

Papanicolaou's solution 3a polychromatic solution EA 31

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109272

Papanicolaou's solution 3b polychromatic solution EA 50

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115938

Hematoxylin monohydrate (C.I.75290)

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Accessories (Secondary)

Catalogue Number

Description

104302

Hematoxylin cryst. (C.I. 75290)

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105175

Hematoxylin solution modified acc. to Gill II

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106887

Papanicolaou’s solution 2b Orange II solution

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106888

Papanicolaou's solution 2a Orange G solution (OG 6)

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109254

Papanicolaou's solution 1b Hematoxylin solution S

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109271

Papanicolaou's solution 3a polychromatic solution EA 31

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109272

Papanicolaou's solution 3b polychromatic solution EA 50

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109253 Sigma-AldrichPapanicolaou's solution 1a Harris' hematoxylin solution

for cytology

Synonyms: Hematoxylin solution acc. to Harris, Colour solution acc. to Papanicolaou, Harris' Hematoxylin solution

Recommended Products

Overview

Pricing & Availability

Documentation

Papanicolaou's solution 1a Harris' hematoxylin solution SDS

Papanicolaou's solution 1a Harris' hematoxylin solution Certificates of Analysis

User Guides

Related Products & Applications

Accessories (Primary)

Accessories (Secondary)

Related Products By: Application Facete

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