Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Millicell® Cell Culture Inserts
Optimized cell growth, attachment and differentiationMore
Optimized cell growth, attachment and differentiation Less
With Millicell inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell inserts make it possible to study both sides of the cell monolayer.
Millicell inserts are available for 24-, 12-, or 6-well plates. The inserts are easily prepared for SEM and TEM visualizing techniques, and they are compatible with cellular and/or fluorescent stains.
Insert Formats
Millicell Hanging Inserts
For co-culturing and permeability assays
Unique design allows easier basolateral access than other hanging inserts with less risk of contamination
Available in 5 pore sizes and 3 diameters, including a 1 µm pore size that is optically transparent for better visualization by microscopy
NEW: Available preloaded in 24-well receiver plates or as individual inserts in 3 diameters
Millicell Standing Inserts
Promotes excellent cell growth and provides an exceptional opportunity for cell studies
Available with Biopore (PTFE) membrane, MF-Millipore (mixed cellulose esters) membrane, and polycarbonate membrane
Millicell Organotypic Insert
For high cell viability and superior study of three dimensional explant structure
Lower height allows them to fit inside a standard petri dish
The Biopore™ (PTFE) membrane provides high viability—for as long as 40 days—and excellent trans-membrane oxygen transport
The membrane is optically clear and optimized for long-term organotypic explant maintenance
Membrane Types
Biopore Membrane (hydrophilic PTFE)
For low protein-binding, live cell viewing, and immunofluorescent applications
MF-Millipore™ Membrane (mixed cellulose esters)
For exceptional anatomical and functional polarization
Isopore™ Membrane (polycarbonate)
For growth of attachment-dependent cells without matrix
PET Membrane (polyethylene terephthalate)
For growth of attachment-dependent cells without matrix
With Millicell® inserts, adherent or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.
Propagation of human embryonic stem cells in a microporous membrane-based indirect co-culture system Kelsey Albert, Steven Sheridan, Louise Laurent, Igor Ulitsky, Ron Shamir, Jeanne Loring, & Raj R. Rao Biochemical and Biophysical Research Communications
2010
Astrocyte growth effects of vascular endothelial growth factor (VEGF) application to perinatal neocortical explants: Receptor mediation and signal transduction pathways Nina Mani, Alfia Khaibullina, Janette Krum and Jeffrey Rosenstein Experimental Neurology 192 (2005); 394-406
2005
Cell Culture
Subcellular localisation of recombinant a and g-synuclein Christian Specht, Cezar Tigaret, George Rast, Agnea Thalhammer, York Rudhard and Ralf Schoepfer Mol. Cell. Neurosci., 28 (2005); 326-334
2005
Cell Culture
Establishment of the organotypic model of amyotrophic lateral sclerosis from the SD rats' spinal cord Diao ZY, et. al,Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese. Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese.
2005
Cell Culture
Neural stem cells protect against glutamate-induced excitotoxicitiy and promote survival of injured motor neurons through the secretion of neurotrophic factors Jeronia Llado, Christine Haenggeli, Nicholas Maragakis, Evan Snyder and Jeffrey Rothstein Mol. Cell. Neurosci. , 27 (2004); 322-331
2004
Cell Culture
Development of an in vitro blood-brain barrier model-cytotoxicity of mercury and aluminum. Toimela, T et. al.,Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82. Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82.
2004
Morphological differentiation of bone marrow stromal cells into neuron-like cells after co-culture with hippocampal slice Abouelfetouh, Ayman, et al Brain Research (2004), Volume 1029, Issue 1 pp 114-119
2004
Cell Culture
Effect of sodium bicarbonate on extracellular pH, matrix accumulation, and morphology of cultured articular chondrocytes. Waldman SD, Couto DC, Omelon SJ, Kandel RA Tissue Engineering (2004), Nov-Dec;10(11-12):1633-40
2004
FGF-10 plays an essential role in the growth of the fetal prostate. Annemarie A. Donjacour, Axel A. Thomson and Gerald R. Cunha Developmental Biology 261 (1): 39-54
2003
Cell Culture
Changes in lymphokine receptor expression and fatty acid composition of phospholipids and triacylglycerols in rat adipocytes associated with lymph nodes following a transient immune challenge. J. D. Priddle, C. A. Mattacks, D. A. Sadler, H. A. MacQueen and C. M. Pond Cell Biology International 27 (1): 23-29
2003
Are the Millicell PCF inserts tissue culture treated on both sides of the membrane?
Yes, they are tissue culture treated on both sides of the membrane. This allows for attachment- dependent cell culture on both sides of the membrane.
Are the Millicell units sterile and, if so, how are they sterilized?
The Millicell units are sold sterile in individual blister packs. The HA and CM units are ETO sterilized and the PC and PCF are Gamma Irradiated.
Can cells grown on Isopore (polycarbonate) membrane be viewed under a microscope?
Yes, we can observe the cells seeding on the membrane of pore size 1 um.
Can I make membrane potential measurements with the Millicell-ERS?
Yes, membrane potential measurements can be made with the ERS. Short circuit potential, however, cannot be done.
How can I be sure that I have a confluent monolayer in my Millicell ?
There are several answers to this question based on the type of membrane you are using and the equipment you have. It is possible to view, via phase contrast microscopy, actual cell layers if you are using Millicell HA, PC and PCF.
With Millicell CM you can use bright field microscopy as well to check for confluency. Also, one way of assuring confluency is to seed your cells at optimal levels. Most cell lines do best at a density of 5x10^5 to 1x10^6 cells/ cm2. So long as you are working with a cell type (i.e. epithelial cells) that exhibits resistance due to membrane potential you can also use the Millicell ERS to confirm confluency.
How long do the electrodes last?
The electrodes will last at least 6 months with normal use and care, barring any physical damage. To prolong life of the electrodes, clean after every use and store dry.
How should I store electrodes when not in use?
When storing electrodes for long periods of time, wash the electrodes with Milli-Q water or equivalent to remove salts and proteins, then store dry. For short term storage electrodes can be stored in a buffered solution. Make sure the electrode cable plug is connected to the electrode port on the Millicell ERS meter so that the system is internally short-circuited and electrode symmetry is maintained.
I am coating the Millicell CM with Rat Tail collagen according to your instructions and sometimes I am seeing an empty ring at the periphery of the insert where the collagen has not filled in and an empty "hole" at the center where there is no coating either. How can I avoid this from happening in the future?
If you are using Type 1 collagen, it should be filter sterilized in non-denaturing alcohol prior to use in the Millicell. Also, try looking at the insert while you are coating it and tap/rotate it to enhance uniform liquid coating. The entire membrane should go clear which indicates uniform wetting/coverage of the Millicell. Also, be sure to use at least minimum volumes of 50ul for the 12mm and 350 ul per 30 mm.
I am concerned about the possibility of introducing surfactants in cell cultures using the Millicell PCF product. Is the Polycarbonate membrane in Millicell-PCF PPVP (surfactant) free?
Yes, the Polycarbonate membrane in Millicell-PCF is PPVP (surfactant) free.
I am culturing tissue slices on the Millicell Polycarbonate unit (PIHP01250) and it appears that the media is not being retained by the unit. What is going on?
The 0.4 um PCF polycarbonate membrane is tissue culture treated and, therefore, hydrophilic. In long-term culture ( >than a few to 24 hours) they will NOT retain fluid since it will slowly pass through the pores. A monolayer of epithelial cells on the membrane serves as a secondary barrier which will selectively prevent/or allow molecules to pass.
If you are referring to an overnight culture with no media outside the Millicell this result is to be expected. If there is equal fluid heights both inside and outside the Millicell ( ~ 400 ul inside and 600 ul outside) then the fluid will be retained. Since they are doing organotypic culture you could easily adjust the inside and outside volumes to the same desire height to make sure the culture remains bathed in media.
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