Millipore Sigma Vibrant Logo

426100 Lactacystin, Synthetic - CAS 1258004-00-0 - Calbiochem

Lactacystin, Synthetic, CAS 1258004-00-0, is an irreversible inhibitor of 20S proteasome inhibitor (IC₅₀ = 500 nM). A covalent inhibitor of the chymotrypsin & trypsin-like activities of proteasome.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Proteasome Inhibitor VI

Primary Target: 20S proteasome (MCP; multicatalytic proteinase complex)
View Products on Sigmaaldrich.com
426100
View Pricing & Availability

Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
1258004-00-0C₁₅H₂₄N₂O₇S

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
426100-200UG
Retrieving availability...
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Plastic ampoule 200 μg
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      Description
      OverviewAn irreversible proteasome inhibitor. A Streptomyces metabolite that acts as a highly specific inhibitor of the 20S proteasome (MCP; multicatalytic proteinase complex) (IC50 = 500 nM for 20S proteasome). Blocks proteasome activity by targeting the catalytic β-subunit. Acts as a covalent inhibitor of the chymotrypsin- and trypsin-like activities of the proteasome. Induces neurite outgrowth in Neuro 2A mouse neuroblastoma cells and inhibits progression of synchronized Neuro 2A cells and MG-63 human osteosarcoma cells beyond the G1 phase of the cell cycle. Reported to induce apoptosis in human monoblast U937 cells. Also inhibits NF-κB activation (IC50 = 10 µM).
      Catalogue Number426100
      Brand Family Calbiochem®
      SynonymsProteasome Inhibitor VI
      References
      ReferencesKeyomarsi, K., et al. 2011. Cell. Biol. Toxicol. 27, 123.
      Adams, J., and Stein, R. 1996. Ann. Rep. Med. Chem. 31, 279.
      Dick, L.R., et al. 1996. J. Biol. Chem. 271, 7273.
      Oda, K., et al. 1996. Biochem. Biophys. Res. Commun. 219, 800.
      Fenteany, G., et al. 1995. Science 268, 726.
      Imajoh-Ohmi, S., et al. 1995. Biochem. Biophys. Res. Commun. 217, 1070.
      Jensen, T.J., et al. 1995. Cell 83, 129.
      Katagiri, M.M., et al. 1995. J. Antibiot. 48, 344.
      Mori, S., et al. 1995. J. Biol. Chem. 270, 29447.
      Tanaka, H., et al. 1995. Biochem. Biophys. Res. Commun. 216, 291.
      Fenteany, G., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 3358.
      Omura, S., et al. 1991. J. Antibiot. 44, 117.
      Product Information
      CAS number1258004-00-0
      ATP CompetitiveN
      FormWhite solid
      Hill FormulaC₁₅H₂₄N₂O₇S
      Chemical formulaC₁₅H₂₄N₂O₇S
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary Target20S proteasome (MCP; multicatalytic proteinase complex)
      Primary Target IC<sub>50</sub>500 nM against 20S proteasome; 10 µM against NF-κB activation
      Purity≥99% by HPLC
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution in DMSO, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month at -20°C. Unstable in aqueous solutions; reconstitute in H₂O just prior to use.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      426100-200UG 04055977187328

      Documentation

      Lactacystin, Synthetic - CAS 1258004-00-0 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Lactacystin, Synthetic - CAS 1258004-00-0 - Calbiochem Certificates of Analysis

      TitleLot Number
      426100

      References

      Reference overview
      Keyomarsi, K., et al. 2011. Cell. Biol. Toxicol. 27, 123.
      Adams, J., and Stein, R. 1996. Ann. Rep. Med. Chem. 31, 279.
      Dick, L.R., et al. 1996. J. Biol. Chem. 271, 7273.
      Oda, K., et al. 1996. Biochem. Biophys. Res. Commun. 219, 800.
      Fenteany, G., et al. 1995. Science 268, 726.
      Imajoh-Ohmi, S., et al. 1995. Biochem. Biophys. Res. Commun. 217, 1070.
      Jensen, T.J., et al. 1995. Cell 83, 129.
      Katagiri, M.M., et al. 1995. J. Antibiot. 48, 344.
      Mori, S., et al. 1995. J. Biol. Chem. 270, 29447.
      Tanaka, H., et al. 1995. Biochem. Biophys. Res. Commun. 216, 291.
      Fenteany, G., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 3358.
      Omura, S., et al. 1991. J. Antibiot. 44, 117.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Proteasomes Technical Bulletin

      Citations

      Title
    • Keyomarsi, K., et al. 2011. Cell. Biol. Toxicol. 27, 123.
    • Lacher, M.D., et al. 2006. Cancer Research 66, 1648.
    • Wu, C-J., et al. 2005. European Molecular Biology Organization Journal 24, 1886.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-October-2012 JSW
      SynonymsProteasome Inhibitor VI
      DescriptionAn irreversible proteasome inhibitor. A Streptomyces metabolite that acts as a highly specific inhibitor of the 20S proteasome (MCP; multicatalytic proteinase complex) (IC50 = 500 nM for 20S proteasome). Blocks proteasome activity by targeting the catalytic β-subunit. Acts as a covalent inhibitor of the chymotrypsin and trypsin-like activities of the proteasome. Induces neurite outgrowth in Neuro 2A mouse neuroblastoma cells beyond theG1 phase of the cell cycle. Reported to induce apoptosis in human monoblast U937 cells. Also inhibits NF-κB activation (IC50 = 10 µM)
      FormWhite solid
      CAS number1258004-00-0
      Chemical formulaC₁₅H₂₄N₂O₇S
      Structure formulaStructure formula
      Purity≥99% by HPLC
      SolubilityDMSO (50 mg/ml) or H₂O
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution in DMSO, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month at -20°C. Unstable in aqueous solutions; reconstitute in H₂O just prior to use.
      Toxicity Standard Handling
      ReferencesKeyomarsi, K., et al. 2011. Cell. Biol. Toxicol. 27, 123.
      Adams, J., and Stein, R. 1996. Ann. Rep. Med. Chem. 31, 279.
      Dick, L.R., et al. 1996. J. Biol. Chem. 271, 7273.
      Oda, K., et al. 1996. Biochem. Biophys. Res. Commun. 219, 800.
      Fenteany, G., et al. 1995. Science 268, 726.
      Imajoh-Ohmi, S., et al. 1995. Biochem. Biophys. Res. Commun. 217, 1070.
      Jensen, T.J., et al. 1995. Cell 83, 129.
      Katagiri, M.M., et al. 1995. J. Antibiot. 48, 344.
      Mori, S., et al. 1995. J. Biol. Chem. 270, 29447.
      Tanaka, H., et al. 1995. Biochem. Biophys. Res. Commun. 216, 291.
      Fenteany, G., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 3358.
      Omura, S., et al. 1991. J. Antibiot. 44, 117.
      Citation
    • Keyomarsi, K., et al. 2011. Cell. Biol. Toxicol. 27, 123.
    • Lacher, M.D., et al. 2006. Cancer Research 66, 1648.
    • Wu, C-J., et al. 2005. European Molecular Biology Organization Journal 24, 1886.
      		•