Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Description
Overview
Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
Catalogue Number
324715
Brand Family
Calbiochem®
References
References
Jekel, P.A., et al. 1983. Anal. Biochem. 134, 347.
Product Information
CAS number
72561-05-8
Unit of Definition
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-<i>p</i>NA per min at 25°C, pH 7.7.
EC number
3.4.21.50
Form
Lyophilized
Formulation
Lyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.
Harmful by inhalation. Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation and skin contact.
S Phrase
S: 22-26-36
Do not breathe dust. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Harmful
Storage
+2°C to +8°C
Hygroscopic
Hygroscopic
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes Certificates of Analysis
Title
Lot Number
324715
References
Reference overview
Jekel, P.A., et al. 1983. Anal. Biochem. 134, 347.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
01-February-2012 RFH
Description
Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK.
Form
Lyophilized
Formulation
Lyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.
Recommended reaction conditions
Protocol
1. Dissolve the proteins to be sequenced in 25 mM Tris-HCl, pH 8.5, and 1 mM EDTA. For proteins that are difficult to solubilize, add urea, SDS, or guanidine-HCl to the digestion buffer prior to solubilization. If urea is used, add 20 mmol/l of methylamine.
2. The suggested working concentration is 1:20-1:100 (protease:protein by weight).
3. Incubate for 2-18 h at 37°C.
CAS number
72561-05-8
EC number
3.4.21.50
Purity
≥90% by SDS-PAGE
Specific activity
≥200 units/mg protein
Unit definition
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-pNA per min at 25°C, pH 7.7.
Solubility
Reconstitute in 50 µl of distilled H₂O.
Storage
+2°C to +8°C Hygroscopic
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
Toxicity
Harmful
References
Jekel, P.A., et al. 1983. Anal. Biochem. 134, 347.
