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268298 DAPI, Dihydrochloride - CAS 28718-90-3 - Calbiochem

Overview

Key Spec Table

CAS #Empirical Formula
28718-90-3C₁₆H₁₅N₅ · 2HCl

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268298-10MG
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      Description
      OverviewCell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H2O (Cat. No. 508741).
      Catalogue Number268298
      Brand Family Calbiochem®
      Synonyms4ʹ,6-Diamidino-2-phenylindole, 2HCl
      References
      ReferencesMathieu, C., et al. 1995. Exp. Cell Res. 216, 13.
      Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
      Hotz, M.A., et al. 1994. Cytometry 15, 237.
      Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
      Miller, T., et al. 1993. Biotechniques 15, 1042.
      Gray, J.W., et al. 1982. Science 238, 323.
      Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315.
      Russels, W.C., et al. 1975. Nature 253, 461.
      Product Information
      CAS number28718-90-3
      ATP CompetitiveN
      FormYellow solid
      Hill FormulaC₁₆H₁₅N₅ · 2HCl
      Chemical formulaC₁₆H₁₅N₅ · 2HCl
      Hygroscopic Hygroscopic
      ReversibleY
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Biological Information
      Primary TargetBinds preferentially to DNA rich in adenine and thymine
      Purity≥90% by HPLC
      Physicochemical Information
      Cell permeableN
      Emission max.
      Excitation max.
      Safety Information according to GHS
      RTECSNL5995050
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.
      Global Trade Item Number
      Catalogue Number GTIN
      268298-10MG 04055977197785