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382061 Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem

Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
23491-45-4C₂₅H₂₄N₆O · 3HCl

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382061-100MG
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      Plastic ampoule 100 mg
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      Description
      OverviewMembrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.
      Catalogue Number382061
      Brand Family Calbiochem®
      Synonyms2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)-benzimidazole, 3HCl, HOE 33258
      References
      ReferencesWerstuck, G., and Green, M.R. 1998. Science 282, 296.
      Battaglio, M., et al. 1994. Biotech. Histochem. 69, 152.
      Elstein, K.H., and Zucker, R.M. 1994. Exp. Cell Res. 211, 322.
      Tarshis, M., et al. 1994. J. Immunol. Methods 168, 245.
      Beeler, T.J., and Gable, K. 1993. J. Membr. Biol. 135, 109.
      Oberhammer, F., et al. 1993. EMBO J. 12, 3679.
      Papadimitriou, E., and Lelkes, P.I. 1993. J. Immunol. Methods 162, 41.
      Jin, R., and Breslauer, K.J. 1988. Proc. Natl. Acad. Sci. USA 85, 8939.
      Cesarone, C.F., et al. 1979. Anal. Biochem. 100, 188.
      Latt, S.A., and Stetten, G. 1976. J. Histochem. Cytochem. 24, 24.
      Product Information
      CAS number23491-45-4
      ATP CompetitiveN
      FormYellow-green solid
      Hill FormulaC₂₅H₂₄N₆O · 3HCl
      Chemical formulaC₂₅H₂₄N₆O · 3HCl
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetAdenine-thymine-specific fluorescent stain
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSSM1140500
      Safety Information
      R PhraseR: 36/37/38-40

      Irritating to eyes, respiratory system and skin.
      Limited evidence of a carcinogenic effect.
      S PhraseS: 22-26-36

      Do not breathe dust.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Carcinogenic / Teratogenic
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 2 weeks at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      382061-100MG 04055977213065

      Documentation

      Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem Certificates of Analysis

      TitleLot Number
      382061

      References

      Reference overview
      Werstuck, G., and Green, M.R. 1998. Science 282, 296.
      Battaglio, M., et al. 1994. Biotech. Histochem. 69, 152.
      Elstein, K.H., and Zucker, R.M. 1994. Exp. Cell Res. 211, 322.
      Tarshis, M., et al. 1994. J. Immunol. Methods 168, 245.
      Beeler, T.J., and Gable, K. 1993. J. Membr. Biol. 135, 109.
      Oberhammer, F., et al. 1993. EMBO J. 12, 3679.
      Papadimitriou, E., and Lelkes, P.I. 1993. J. Immunol. Methods 162, 41.
      Jin, R., and Breslauer, K.J. 1988. Proc. Natl. Acad. Sci. USA 85, 8939.
      Cesarone, C.F., et al. 1979. Anal. Biochem. 100, 188.
      Latt, S.A., and Stetten, G. 1976. J. Histochem. Cytochem. 24, 24.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-June-2015 JSW
      Synonyms2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)-benzimidazole, 3HCl, HOE 33258
      DescriptionMembrane-permeant, adenine-thymidine specific fluorescent stain (excitation maximum: 346 nm; emission maximum: 460 nm). Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5' untranslated region (UTR) to control expression of transfected gene constructs.
      FormYellow-green solid
      CAS number23491-45-4
      RTECSSM1140500
      Chemical formulaC₂₅H₂₄N₆O · 3HCl
      Structure formulaStructure formula
      Purity≥98% by HPLC
      SolubilityH₂O (20 mg/ml). Precipitates in phosphate buffered solutions
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 2 weeks at 4°C.
      Toxicity Carcinogenic / Teratogenic
      ReferencesWerstuck, G., and Green, M.R. 1998. Science 282, 296.
      Battaglio, M., et al. 1994. Biotech. Histochem. 69, 152.
      Elstein, K.H., and Zucker, R.M. 1994. Exp. Cell Res. 211, 322.
      Tarshis, M., et al. 1994. J. Immunol. Methods 168, 245.
      Beeler, T.J., and Gable, K. 1993. J. Membr. Biol. 135, 109.
      Oberhammer, F., et al. 1993. EMBO J. 12, 3679.
      Papadimitriou, E., and Lelkes, P.I. 1993. J. Immunol. Methods 162, 41.
      Jin, R., and Breslauer, K.J. 1988. Proc. Natl. Acad. Sci. USA 85, 8939.
      Cesarone, C.F., et al. 1979. Anal. Biochem. 100, 188.
      Latt, S.A., and Stetten, G. 1976. J. Histochem. Cytochem. 24, 24.