Millipore Sigma Vibrant Logo
 
Life Science Research
 

RIP

 Request Information
Featured Literature
Merck:/Freestyle/BI-Bioscience/Antibodies-Assays/epigenetics-images/AN1327EN00_EM-1.jpgEffective Nuclear RIP Protocols to Study Interactions between Proteins
Demonstrates that RIP-based protocols and procedures can be effectively optimized for the isolation and detection of protein-associated long non-coding RNAs (lncRNAs) in chromatin.

Download PDF Here


Exploring the RNA world white paper, download your copy here.Exploring the RNA world:
An introduction to genome-wide analysis of RNA-protein interactions using RNA-binding protein Immuno -precipitation (RIP).

Download PDF Here

RNA-Binding Protein Immunoprecipitation (RNA RIP)

RNA-binding protein immunoprecipitation (RIP) is the RNA analog of the more well-known ChIP application (chromatin immunoprecipitation). RIP can be used to identify specific RNA molecules (of many types) associated with specific nuclear or cytoplasmic binding proteins. These experiments involve immunoprecipitation of endogenously formed complexes of RNA-binding proteins and co-isolation of any RNA species associated with that RNA-binding protein. Purification of these RNA species allows interrogation and identification of mRNAs (and potentially non-coding RNAs associated with them) and can be directly measured using down stream applications including quantitative reverse transcription polymerase chain reaction (qRT-PCR), microarray analysis (RIP-chip) and "deep-sequencing" or 2nd-generation sequencing based platforms (RIP-Seq).

General RIP workflow
Click image to enlarge

Non-coding RNA (ncRNA) Kits

Our RIP immunoprecipitation kit allows researchers to perform RIP experiments using antibodies directed against RNA-binding proteins or epitope tags. Additionally, we offer RIP validated antibodies available separately in kit type format including controls useful for validation of RIP experiments, designated RIPAb+ kits

Magna ChIRP™ Kits

The Magna ChIRP™ RNA Interactome Kit uses the ChIRP method (Chromatin Isolation by RNA Purification) to enable identification and analysis of regions of chromatin that interact with a particular chromatin-associated RNA. This novel anti-sense probe-based capture strategy uses cross-linked chromatin to allow unbiased discovery of RNA-associated DNA sequences and proteins.

Magna Nuclear RIP™ Kits

Magna Nuclear RIP™ Kits are specially designed to allow the discovery and analysis of a variety of chromatin associated RNAs such as long non-coding RNAs, enhancer RNAs and miRNAs . These chromatin-associated RNAs often regulate gene expression and can be analyzed with applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and next generation sequencing (RIP-Seq).

Magna RIP™ Kits

Magna RIP™ Kits make this powerful technique accessible to everyone, regardless of prior experience with immunoprecipitation or RNA-based procedures. Fully compatible with a wide range of RIP validated antibodies, and contains all reagents needed for robust, specific enrichment of RBP-associated RNAs.

Magna MeRIP™ m6A Kit

The Magna MeRIP m6A kit utilizes the MeRIP (methylated RNA immunoprecipitation) method to enable identification and transcriptome-wide profiling of m6A RNA modifications. In the MeRIP assay, RNA is chemically fragmented into 100 nucleotides or smaller fragments followed by magnetic immunoprecipitation with a monoclonal antibody toward m6A. After immunoprecipitation, isolated RNA fragments can be subjected with qRT-PCR or RNA sequencing (MeRIP-seq).