Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Histone modifications regulate DNA transcription, repair, recombination, and replication, and can alter local chromatin architecture. Understanding histone modifications is key to uncovering epigenetic mechanisms of gene regulation. Merck grasps the complexity of this research and provides a growing line of kits, antibodies, and assays for studying histones and their modifications. Merck offers over 600 validated antibodies, recombinant proteins, and kits to analyze histone and histone-variant phosphorylation, methylation, acetylation, ubiquitination, and citrullination-the most widely studied histone modifications in epigenetics today.
We provide bulk reagents Proteins and Enzymes along with our bulk reagents, across a range of subcategories from Cytokines and Growth Factors to Substrate Proteins and Peptides.
Cytoplasmic signaling proteins play crucial roles in cell cycle, targeted proteolysis, protein trafficking, cytoskeletal organization, and gene expression.
Chromatin is a complex molecule that can be found in either an open decondensed or active state (euchromatin) or in a tightly compacted inactive state (heterochromatin). Merck also offers a wide selection of ChIP-qualified antibodies against modified and unmodified histones, transcription factors, chromatin modifying proteins and other key chromatin associated proteins.
Cleavage enzymes were found to cut DNA at specific sites, creating unique, gene-size fragments that could be fused together in the laboratory. Learn more about our best selling products designed for highly specific cleavage of fusion proteins followed by removal of enterokinase, factor Xa, HRV 3C protease and thrombin.
Chromatin is the complex of genomic DNA and associated proteins in the nucleus. It allows cells to package their DNA, provides a scaffold for cell division, and enables control of gene expression.
Methylation as a part of signal transduction is carried out by methyltransferase enzymes that reversibly add methyl groups to the carboxyl groups of proteins and DNA, forming methyl esters.
Custom beads match your application whether working with recombinant proteins, studying protein interactions, or immobilizing enzymes and nucleic acids.
We offer a selection of assay kits and antibodies to measure acetylation and deacetylation activity, helping to elucidate the relationship between chromatin modification pathways and gene regulation.
DNA methylation is involved in the regulation of many cellular processes, including chromosome stability, chromatin structure, X chromosome inactivation, embryonic development, and transcription. DNA methylation occurs mainly at the 5' position of cytosine bases and is mediated by enzymes called DNA methyltransferases (DNMT). Regions of methylated DNA can be bound by a variety of methylated DNA binding proteins (MBD).