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Recombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag® sequence and expressed in NSO-derived mouse myeloma cells. sFRP-3 is a soluble antagonist of Wnt signaling. It prohibits interactions with Frizzled receptors by binding directly to Wnt proteins.
Catalogue Number
565834
Brand Family
Calbiochem®
References
References
Hoang, et al. 1996. J. Biol. Chem.271, 26131.
Leyns, et al. 1997 Cell88, 747.
Wang, et al. 1997 Cell88, 757.
Mayr, et al. 1997 Mech. Dev.63, 109.
Rattner, et al. 1997. PNAS94, 2859.
Proliferation Assay: ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.
Functional ELISA: 50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
Purity
≥95% by SDS-PAGE
Physicochemical Information
Contaminants
Endotxin: ≤1 EU/µg
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstituion refrigerate (4°C) for short term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Hoang, et al. 1996. J. Biol. Chem.271, 26131.
Leyns, et al. 1997 Cell88, 747.
Wang, et al. 1997 Cell88, 757.
Mayr, et al. 1997 Mech. Dev.63, 109.
Rattner, et al. 1997. PNAS94, 2859.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
21-Sepmtember-2011 RFH
Description
Recombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag® sequence and expressed in NSO-derived mouse myeloma cells. sFRP-3 is a soluble antagonist of Wnt signaling. It prohibits interactions with Frizzled receptors by binding directly to Wnt proteins.
Form
Lyophilized
Formulation
Lyophilized from 0.2 µm filtered PBS, BSA.
Purity
≥95% by SDS-PAGE
Contaminants
Endotxin: ≤1 EU/µg
Biological activity
Proliferation Assay: ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.
Functional ELISA: 50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
Solubility
Reconstitute with sterile PBS contanining ≥1% human or bovine serum albumin. Please refer to the vial label for lot-specific reconstitution volume.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstituion refrigerate (4°C) for short term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Hoang, et al. 1996. J. Biol. Chem.271, 26131.
Leyns, et al. 1997 Cell88, 747.
Wang, et al. 1997 Cell88, 757.
Mayr, et al. 1997 Mech. Dev.63, 109.
Rattner, et al. 1997. PNAS94, 2859.
