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70840 pTriEx™-1.1 DNA - Novagen

70840
Purchase on Sigma-Aldrich

概述

Replacement Information

Products

产品目录编号包装 数量 / 包装
70840-3CN 塑胶安瓿;塑胶针药瓶 20 μg
Description
OverviewThe pTriEx™-1.1 vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector a single recombinant plasmid can be used to test expression in E. coli, insect and vertebrate cells. Transient vertebrate expression is mediated by a hybrid promoter composed of the CMV immediate early enhancer fused to the chicken β-actin promoter. For expression in insect cells, pTriEx-1.1 contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector™ System. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. Expression can be induced in hosts such as NovaBlue by infecting with λCE6, a phage that constitutively expresses T7 RNA polymerase from the λpL and λ>pI promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows IPTG based induction.







This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Catalogue Number70840
Brand Family Novagen®
References
Product Information
DeclarationThis product is not available in Japan.
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
70840-3CN 04055977272635

Documentation

pTriEx™-1.1 DNA - Novagen MSDS

职位

物料安全数据表 (MSDS) 

pTriEx™-1.1 DNA - Novagen 分析证书

标题批号
70840

小册子

标题
Insect Cell Expression

引用

标题
  • Cao, Y., et al. 2008. Molecular Cancer Research 6, 119.
  • Grillo, C., et al. 2007. Journal of Biological Chemistry 282, 10299.
  • Popescu, C.I., et al. 2006. Journal of Biological Chemistry 281, 21682.
  • Cao, Y., et al. 2005. Cancer Research 65, 1124.
  • Popescu, C.I., et al. 2005. Journal of Biological Chemistry 280, 13833.
  • Branza-Nichita, N., et al. 2004. Biochemical and Biophysical Research Communications 319, 655.
  • Wurm, T., et al. 2001. Journal of Virology 75, 9345.
  • 用户协议

    标题
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB250 pTriEx™ System Manual

    载体图

    标题
    TB280 pTriEx™-1.1 Vector Map

    载体序列

    标题
    pTri-Ex-1.1 Sequence

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