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70174 pT7Blue Blunt Vector (linearized) - Novagen

pT7Blue Blunt Vector (linearized) - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70174
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概述

Replacement Information

Products

产品目录编号包装 数量 / 包装
70174-3CN 塑胶安瓿;塑胶针药瓶 20 rxn
70174-4CN 塑胶安瓿;塑胶针药瓶 40 rxn
Description
Overview

The pT7Blue vector features the pUC19 backbone (including high-copy number origin of replication and lac sequences), a T7 promoter, f1 origin of replication, and modified multiple cloning region. The multiple cloning region contains an EcoR V site used for T-cloning flanked by an Nde I site, which allows inserts to be conveniently subcloned into the Nde I site of many pET vectors.
Catalogue Number70174
Brand Family Novagen®
Features and benefits
  • No restriction enzymes or special primers
  • Compatible with any DNA polymerase
  • Independent of 3ʹ-dA addition or sequence context of DNA ends
  • Blue/white screening with seven different vectors, including pETBlue expression vectors
  • Simple protocol takes less than one hour* from PCR product to plating transformants

* All times listed refer to kits using NovaBlue Singles™ Competent Cells and ampicillin or chloramphenicol selection. Giga kits, which use NovaBlue GigaSingles™ Competent Cells, require one hour for outgrowth.
References
References

Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.
Product Information
Components
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
70174-3CN 04055977258363
70174-4CN 04055977258370

Documentation

pT7Blue Blunt Vector (linearized) - Novagen 分析证书

标题批号
70174

参考

参考信息概述

Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.

引用

标题
  • Ekaterina Bogdanova, et al. (2008) Transcription regulation of the type II restriction-modification system AhdI. Nucleic Acids Research 36, 1429-1442.
  • Miho Hirabayashi, et al. (2005) Transformation of skeletal muscle from fast- to slow-twitch during acquisition of cold tolerance in the chick. Endocrinology 146, 399-405.
  • J. K. Jang, T. Rahman and K. S. McKim. (2005) The kinesinlike protein subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes. 16, 4684-4694.
  • Rahul Pal, et al. (2005) Evidence for multiple shared antigenic determinants within Ro60 and other lupus-related ribonucleoprotein autoantigens in human autoimmune responses. Journal of Immunology 175, 7669-7677.
  • Dana R. Rundle, et al. (2004) Myristoyl-CoA: protein N-myristoyltransferases: isoform identification and gene expression in retina. Molecular Vision 10, 177-185.
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    用户协议

    标题
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB183 Perfectly Blunt® Cloning Kits

    载体图

    标题
    TB017VM pT7Blue Vector Map

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    种类

    Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Cloning > Cloning Vectors