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69076 pET-32 Ek/LIC Vector Kit - Novagen

69076
Purchase on Sigma-Aldrich

概述

Replacement Information

Products

产品目录编号包装 数量 / 包装
69076-3CN 玻璃瓶 20 rxn
Description
OverviewThe pET-32 Ek/LIC vector is an Ek/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag™ thioredoxin protein, and His•Tag® and S®Tag™ coding sequences that are cleavable with enterokinase (Ek) protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Ek protease cleavage site (AspAspAspAspLys↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence is compatible with purification, detection, and quantification.




Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number69076
Brand Family Novagen®
Application Data
References
Product Information
Components
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
69076-3CN 04055977259537

Documentation

pET-32 Ek/LIC Vector Kit - Novagen 分析证书

标题批号
69076

小册子

标题
Radiance! Multisystem Protein Expression

引用

标题
  • Xuezhi Bi, Gurdev S. Khush and John Bennett. (2005) The rice nucellin gene orthologue, OsAsp1 which encodes an active aspartic protease without plant-specific insert, is strongly expressed in early embryo. Plant and Cell Physiology 46, 87-98.
  • Hamid Mehenni, et al. (2005) LKB1 interacts with and phosphorylates PTEN: a functional link between two proteins involved in cancer predisposing syndromes. Human Molecular Genetics 14, 2209-2219.
  • John V. McDowell, et al. (2004) Demonstration of the involvement of outer surface protein E coiled coil structural domains and higher order structural elements in the binding of infection-induced antibody and the complement-regulatory protein, factor H. Journal of Immunology 173, 7471-7480.
  • 用户协议

    标题
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual
    TB163 Ek/LIC Cloning Kits
    TB184 Xa/LIC Cloning Kits

    载体图

    标题
    TB161VM pET-32 Ek/LIC Vector Map