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506114 p34cdk1/Cyclin B, Human, Recombinant, S. frugiperda

506114
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概述

Replacement Information

Products

产品目录编号包装 数量 / 包装
506114-200UCN 塑胶安瓿;塑胶针药瓶 200 u
Description
OverviewRecombinant, human p34Cdk1/cyclin B expressed in S. frugiperda insect cells. This kinase is a major regulator of the eukaryotic cell cycle. As a monomer, Cdk1 is inactive and requires association with cyclin B and phosphorylation of Thr161 to form an active complex. MW: 34000(p34Cdk1) and 55000(cyclinB).
Catalogue Number506114
Brand Family Calbiochem®
References
ReferencesKobayashi, H., et al. 1994. J. Biol. Chem. 269, 29153.
Atherton-Fessler, S., et al. 1993. Mol. Cell. Biol. 13, 1675.
Product Information
Activity≥20,000 U/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that catalyzes the transfer of 1 pmol of phosphate to histone H1 (10 µM) in 1 min at 30°C, pH 7.5.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM EDTA, 50% glycerol, 0.01% BRIJ® 35 Detergent, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
ContaminantsProtease, phosphatase: none detected
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
506114-200UCN 04055977199444

Documentation

p34cdk1/Cyclin B, Human, Recombinant, S. frugiperda MSDS

职位

物料安全数据表 (MSDS) 

p34cdk1/Cyclin B, Human, Recombinant, S. frugiperda 分析证书

标题批号
506114

参考

参考信息概述
Kobayashi, H., et al. 1994. J. Biol. Chem. 269, 29153.
Atherton-Fessler, S., et al. 1993. Mol. Cell. Biol. 13, 1675.
数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision15-August-2008 RFH
DescriptionRecombinant, human p34Cdk1/cyclin B expressed in S. frugiperda insect cells. This kinase is a major regulator of the eukaryotic cell cycle. As a monomer, p34cdc2 is inactive and requires association with cyclin B to form an active complex.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM EDTA, 50% glycerol, 0.01% BRIJ® 35 Detergent, pH 7.5.
Recommended reaction conditions50 mM Tris-HCl, 10 mM MgCl2, 2 mM DTT, 1 mM EGTA, 100 µM ATP, 0.01% BRIJ® 35 detergent, pH 7.5.
ContaminantsProtease, phosphatase: none detected
Activity≥20,000 U/ml
Unit definitionOne unit is defined as the amount of enzyme that catalyzes the transfer of 1 pmol of phosphate to histone H1 (10 µM) in 1 min at 30°C, pH 7.5.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesKobayashi, H., et al. 1994. J. Biol. Chem. 269, 29153.
Atherton-Fessler, S., et al. 1993. Mol. Cell. Biol. 13, 1675.