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42600 Ultrafree-DA 离心超滤管

Polypropylene, 0.5 mL volume, non-sterile, for DNA extraction

Ultrafree-DA 离心超滤管 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
42600
50  
Purchase on Sigma-Aldrich

概述

Replacement Information
描述
产品目录编号42600
商名
  • Ultrafree
描述Ultrafree-DA 离心超滤管
概述Recover 100 to 10,000 bp DNA from agarose gel slices in a single 10-minute spin. The kit consists of a pre-assembled filter device with an agarose gel nebulizer, a microcentrifuge vial, and modified TAE gel extraction buffer (dry).

The device utilizes gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.

Prepared DNA requires no further purification for most applications, including cloning and radioisotopic or fluorescent DNA sequencing. Since agarose gel electrophoresis has high resolving power, the small and large non-specific amplification products that frequently interfere with cloning and sequencing after PCR are completely removed from the product.

  • Minimal hands-on time
  • 10-minute spin
  • Fully-functional DNA


Performance
Typical DNA Recoveries from Agarose Gels

DNA Size (bp) Percent DNA Recoveries

100 78
700 71
1000 77
2027 47
4361 35
9416 32
23130 29

Protocol
1. Excise the band of interest after routine electrophoretic analysis of PCR products or other DNA through an agarose gel.
2. Place the gel slice into the pre-assembled device. Note: Protocol is not compatible with low melting point agarose.
3. Spin at 5,000 x g for 10 minutes to extract gel slurry into filter cup.
4. DNA can be stored in the capped filtrate vial after discarding the sample filter cup and gel nebulizer.
参考
产品信息
HS代码8421 29 90
色码蓝色
最高工作温度50 °C
过滤器类型微孔滤膜
Quality LevelMQ400
应用
应用Polypropylene, 0.5 mL volume, non-sterile, for DNA extraction
主要应用
  • DNA Extraction
应用说明固定角度 Microfuge 转子，最大离心力为 5,000 x g
生物信息
无菌性非无菌
理化信息
孔径Not Applicable
离心应用DNA Gel Extraction
尺寸
长度45 mm
直径10.7 mm
过滤面积0.2 cm²
体积0.5 mL
材料信息
化学
  • Polypropylene (PP)
装置材料
  • Polypropylene
毒性学信息
GHS安全信息
安全信息
产品使用声明
储存和货运信息
包装信息
数量50
运输信息
补充信息
规格
Global Trade Item Number
产品目录编号 GTIN
42600 04053252743214