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662065 Ubiquitin, Methylated, Human, Recombinant

Ubiquitin, Methylated, Human, Recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

同义词: Me-Ub

662065
Purchase on Sigma-Aldrich

概述

Replacement Information

Products

产品目录编号包装 数量 / 包装
662065-1MGCN 塑胶安瓿;塑胶针药瓶 1 mg
Description
OverviewRecombinant, human methylated ubiquitin. A derivative of ubiquitin in which lysine residues are reductively methylated. Methylated ubiquitin can ligate to protein substrates, but does not form polyubiquitin chains. Protein breakdown rates obtained with methylated ubiquitin are slower than those obtained with unmethylated ubiquitin. Useful for reducing the polyubiquitin chain length and for determining rates of ubiquitin conjugation.
Catalogue Number662065
Brand Family Calbiochem®
SynonymsMe-Ub
References
ReferencesHershko, A. and Heller, H. 1991. J. Biol. Chem. 266, 16376.
Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun. 128, 1079.
Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz. 91, 570.
Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem. 106, 186.
Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem. 254, 4359.
Product Information
FormLyophilized solid
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
662065-1MGCN 04055977261127

Documentation

Ubiquitin, Methylated, Human, Recombinant MSDS

职位

物料安全数据表 (MSDS) 

Ubiquitin, Methylated, Human, Recombinant 分析证书

标题批号
662065

参考

参考信息概述
Hershko, A. and Heller, H. 1991. J. Biol. Chem. 266, 16376.
Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun. 128, 1079.
Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz. 91, 570.
Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem. 106, 186.
Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem. 254, 4359.
数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-September-2008 RFH
SynonymsMe-Ub
DescriptionRecombinant, human methylated ubiquitin. A derivative of ubiquitin in which lysine residues are reductively methylated. Methylated ubiquitin can ligate to protein substrates, but does not form polyubiquitin chains. Protein breakdown rates obtained with methylated ubiquitin are slower than those obtained with unmethylated ubiquitin. Useful for reducing the polyubiquitin chain length and for determining rates of ubiquitin conjugation.
FormLyophilized solid
Recommended reaction conditionsTypical concentrations for non-rate-limiting support of in vitro conjugation reactions range from 200 µM to 1 mM depending on the reaction conditions.
Purity≥95% by SDS-PAGE
SolubilityAqueous buffers (50 mg/ml)
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesHershko, A. and Heller, H. 1991. J. Biol. Chem. 266, 16376.
Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun. 128, 1079.
Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz. 91, 570.
Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem. 106, 186.
Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem. 254, 4359.