Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Native trypsin from bovine pancreas. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis. Designed for protein sequencing or sequence verification, analysis of protein structural domains and cleavage of fusion proteins.
Catalogue Number
650211
Brand Family
Calbiochem®
References
Product Information
CAS number
9002-07-7
Unit of Definition
1 unit is the amount of enzyme that will convert 1 µmol of substrate per min at 37°C in 100 mM Tris pH 8.5
Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation.
S Phrase
S: 22-24-26-36/37
Do not breathe dust. Avoid contact with skin. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing and gloves.
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Irritant
Storage
+2°C to +8°C
Protect from Moisture
Protect from moisture
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Stock solutions are stable for up to 1 month at -20°C or for up to 1 week at 4°C.
Canadian export regulations
Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
19-May-2008 RFH
Description
Native trypsin from bovine pancreas. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis. Designed for protein sequencing or sequence verification, protein structural domains, and cleavage of fusion proteins.
Form
White solid
Recommended reaction conditions
1:20 to 1:100 (protease:protein by weight) for sequence analysis.
CAS number
9002-07-7
RTECS
YN5075000
EC number
3.4.21.4
Purity
≥90% by HPLC
Contaminants
Chymotrypsin: ≤0.05%
Specific activity
≥80 units/mg protein
Unit definition
1 unit is the amount of enzyme that will convert 1 µmol of substrate per min at 37°C in 100 mM Tris pH 8.5
Solubility
Soluble in HCl (1 mM) or trifluoroacetic acid (0.01%).
Storage
Protect from moisture
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Stock solutions are stable for up to 1 month at -20°C or for up to 1 week at 4°C.
