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650277 Trypsin, Bovine, MS Grade

650277
Purchase on Sigma-Aldrich

概述

Replacement Information

Products

产品目录编号包装 数量 / 包装
650277-1SETCN 玻璃瓶 1 set
Description
OverviewA chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry. A trypsin to protein ratio of 1:100 to 1:20 is recommended for mass spectrometry protocols. Note: 1 SET = 5 X 20 µg of modified trypsin and 1 ml of resuspension buffer.
Catalogue Number650277
Brand Family Calbiochem®
References
ReferencesHenzel, W.J., et al. 1993. PNAS 90, 5011.
Rice, R.H., et al. 1977. Biochim Biophys Acta 492, 316.
Product Information
Unit of DefinitionOne BAEE unit is defined as the ammount of enzyme required to produce a ΔA253 of 0.001 per min in a reaction volume of 3.2 ml at pH 7.6 at 25°C.
FormLyophilized
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥7500 BAEE U/mg
Physicochemical Information
ContaminantsChymostrypsin Activity: <3 BTEE U/mg (one BTEE unit is defined as the amount of enzyme required to hydrolyze 1 µmol BTEE per min at 25°C, pH 7.8.)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38-42

Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation.
S PhraseS: 22-24-26-36/37-45

Do not breathe dust.
Avoid contact with skin.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing and gloves.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-°C). Unused stock solutions are stable for up to 2 weeks at -70°C. Avoid freeze/thaw cycles of solution.
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
650277-1SETCN 07790788052157

Documentation

Trypsin, Bovine, MS Grade MSDS

职位

物料安全数据表 (MSDS) 

Trypsin, Bovine, MS Grade 分析证书

标题批号
650277

参考

参考信息概述
Henzel, W.J., et al. 1993. PNAS 90, 5011.
Rice, R.H., et al. 1977. Biochim Biophys Acta 492, 316.
数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-June-2009 JSW
DescriptionA chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry.
FormLyophilized
Recommended reaction conditions
Materials Provided: • Trypsin, Bovine, MS Grade (Kit Component No. KP31814-20UG): 5 vials, 20 µg each • Trypsin Resuspension Buffer (Kit Component No. KP31815-0.5ML): 1 vial, 0.5 ml Preparation Before Use: Before opening the trypsin centrifuge the tube at 15,000 x g for 5 min. Suspend the MS Grade Trypsin in the Trypsin-Suspension Buffer. Recommended reconstitution concentration 0.5-1 µg/µl. IMPORTANT NOTE: In some cases, after reconstitution, there is insoluble material, which is a non-protein and will not interfere with trypsin activity. If you prefer to remove it, centrifuge the tube briefly and transfer the supernatant to a clean tube. Guidelines for use: For mass spectrometry sequence analysis, MS Grade Trypsin to protein ratio of 1:20 to 1:100 is recommended. Use freshly reconstituted MS Grade Trypsin in any sequence analysis. Any unused trypsin may be stored at -70°C and used within two weeks. Avoid repeated freeze-thaw cycle after re-suspension, which may lead to performance loss. Any available protocol can be used for digestion of proteins with MS Grade Bovine Trypsin.
ContaminantsChymostrypsin Activity: <3 BTEE U/mg (one BTEE unit is defined as the amount of enzyme required to hydrolyze 1 µmol BTEE per min at 25°C, pH 7.8.)
Specific activity≥7500 BAEE U/mg
Unit definitionOne BAEE unit is defined as the ammount of enzyme required to produce a ΔA253 of 0.001 per min in a reaction volume of 3.2 ml at pH 7.6 at 25°C.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-°C). Unused stock solutions are stable for up to 2 weeks at -70°C. Avoid freeze/thaw cycles of solution.
Toxicity Multiple Toxicity Values, refer to MSDS
ReferencesHenzel, W.J., et al. 1993. PNAS 90, 5011.
Rice, R.H., et al. 1977. Biochim Biophys Acta 492, 316.