Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C. Aqueous stock solutions have a half-life of ~4 h at room temperature (20°C).
Mansoor, M.A., et al. 1992. Anal. Biochem.200, 218. Zangerle, L., et al. 1992. J. Neurochem.59, 181. Agarwal, R. and Burke, M. 1991. Arch. Biochem. Biophys.290, 1. Martin, R.L., et al. 1989. J. Biol. Chem.264, 11768. Bonet, A., et al. 1988. Biochem. Biophys. Res. Commun.152, 1. Chinn, P.C. 1985. Fed. Proc.44, 1620.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
03-April-2008 RFH
Synonyms
THIOLYTE® MB Reagent, MBB
Description
Contains a reactive functional group (allylic bromide) that is capable of reacting with a wide variety of nucleophiles to form stable covalent adducts. Reacts extremely rapidly with thiol groups under physiological conditions to yield highly fluorescent products. A labeling reaction is typically carried out at room temperature for 2 to 60 min, pH 7.4, using an equivalent amount (or a small excess) of reagent relative to the amount of thiol. Material is chemically and photolytically stable and shows an absorption maxima at 370 to 385 nm and emits light at 477 to 484 nm (turquoise). Readily penetrates membranes. Useful as an alternative tool in labeling methodology, complementing the use of such well known fluorescent labeling reagents as fluorescein and rhodamine isothiocyanates (whose reactions are slower and yield products that fade relatively fast during irradiation) and fluorescamine (which reacts primarily with amine groups, but whose products also fade relatively rapidly).
Form
Yellow solid
CAS number
71418-44-5
Structure formula
Purity
≥95% by HPLC
Solubility
Acetonitrile (13 mg/ml) or Methanol (25 mg/ml). Recommended stock solution is 13 mg/ml (50 mM) in acetonitrile.
Storage
Protect from light
+15°C to +30°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C. Aqueous stock solutions have a half-life of ~4 h at room temperature (20°C).
Toxicity
Standard Handling
References
Mansoor, M.A., et al. 1992. Anal. Biochem.200, 218. Zangerle, L., et al. 1992. J. Neurochem.59, 181. Agarwal, R. and Burke, M. 1991. Arch. Biochem. Biophys.290, 1. Martin, R.L., et al. 1989. J. Biol. Chem.264, 11768. Bonet, A., et al. 1988. Biochem. Biophys. Res. Commun.152, 1. Chinn, P.C. 1985. Fed. Proc.44, 1620.
