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样品制备

MultiScreen plates offer diverse and versatile formats for a variety of preparative methods.

概述

规格

订货信息

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MultiScreenHTS 滤膜板，内置亲水性 Durapore PVDF 膜清除排序和过滤条件 Show Filter
产品目录编号孔径色码装置材料无菌性包装大小
MSGVN22100.22 µm 透明 苯乙烯 非无菌 10
MSGVN22500.22 µm 透明 苯乙烯 非无菌 50
MSGVS22100.22 µm 透明 苯乙烯 无菌 10
MSGVN2B500.22 µm 不透明 Barex/TiO<sub>2 </sub> 非无菌 50
MSHVN45100.45 µm 透明 苯乙烯 非无菌 10
MSHVN45500.45 µm 透明 苯乙烯 非无菌 50
MSHVN4B500.45 µm 不透明 Barex/TiO<sub>2 </sub> 非无菌 50
MSHVS45100.45 µm 透明 苯乙烯 无菌 10
MSDVN65100.65 µm 透明 苯乙烯 非无菌 10
MSDVN65500.65 µm 透明 苯乙烯 非无菌 50
MSDVN6B500.65 µm 不透明 Barex/TiO<sub>2 </sub> 非无菌 50
MSBVN12101.2 µm 透明 苯乙烯 非无菌 10
MSBVN12501.2 µm 透明 苯乙烯 非无菌 50
MSBVS12101.2 µm 透明 苯乙烯 无菌 10
MSBVN1B501.2 µm 不透明 Barex/TiO<sub>2 </sub> 非无菌 50
MVHVN45250.45 µm 不透明 Polypropylene 非无菌 25

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MultiScreenHTS 多孔滤膜板，内置亲水性 MCE 膜清除排序和过滤条件 Show Filter
产品目录编号孔径色码装置材料无菌性包装大小
MSHAN45100.45 µm 透明 苯乙烯 非无菌 10
MSHAN45500.45 µm 透明 苯乙烯 非无菌 50
MSHAS45100.45 µm 透明 苯乙烯 无菌 10
MSHAN4B500.45 µm 不透明 Barex/TiO<sub>2 </sub> 非无菌 50
MSHAS4B100.45 µm 不透明 Barex/TiO<sub>2 </sub> 无菌 10

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96 孔 MultiScreenHTS 滤膜板，内置玻璃纤维滤膜清除排序和过滤条件 Show Filter
产品目录编号孔径色码装置材料无菌性包装大小
MSFBN6B101.0 / 0.65 µm 不透明 Barex/TiO<sub>2 </sub> 非无菌 10
MSFBN6B501.0 / 0.65 µm 不透明 Barex/TiO<sub>2 </sub> 非无菌 50
MSFCN6B101.2 / 0.65 µm 不透明 Barex/TiO<sub>2 </sub> 非无菌 10
MSFCN6B501.2 / 0.65 µm 不透明 Barex/TiO<sub>2 </sub> 非无菌 50
MVFCN12251.2 µm 不透明 Polypropylene 非无菌 25

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384 孔 MultiScreenHTS 滤膜板，内置玻璃纤维滤膜清除排序和过滤条件 Show Filter
产品目录编号孔径色码装置材料无菌性包装大小
MZFBN0W101.0 / 0.65 µm 不透明 Barex/TiO<sub>2 </sub> 非无菌 10
MZFBN0W501.0 / 0.65 µm 不透明 Barex/TiO<sub>2 </sub> 非无菌 50
MZFCN0W101.2 / 0.65 µm 不透明 Barex/TiO<sub>2 </sub> 非无菌 10
MZFCN0W501.2 / 0.65 µm 不透明 Barex/TiO<sub>2 </sub> 非无菌 50

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MultiScreen 耐溶剂滤膜板，内置亲水性 PTFE 膜清除排序和过滤条件 Show Filter
产品目录编号描述孔径包装大小
MSRLN0410MultiScreen Solvinert，亲水性 PTFE，0.45 µm，无色，非无菌 0.45 µm 10
MSRLN0450MultiScreen Solvinert，亲水性 PTFE，0.45 µm，无色，非无菌 0.45 µm 50
MDRLN0410MultiScreen 深孔 Solvinert，亲水性 PTFE，0.45 µm，无色，非无菌 0.45 µm 10

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MultiScreen 耐溶剂滤膜板，内置疏水性 PTFE 膜清除排序和过滤条件 Show Filter
产品目录编号描述孔径包装大小
MSRPN0410MultiScreen Solvinert，疏水性 PTFE，0.45 µm，无色，非无菌 0.45 µm 10
MSRPN0450MultiScreen Solvinert，疏水性 PTFE，0.45 µm，无色，非无菌 0.45 µm 50
MDRPN0410MultiScreen 深孔 Solvinert，疏水性 PTFE，0.45 µm，无色，非无菌 0.45 µm 10

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Accessories清除排序和过滤条件 Show Filter
产品目录编号描述包装大小
WP6110060Chemical Duty 泵，100 V/50–60 Hz 1
WP6111560Chemical Duty 泵，115 V/60 Hz 1
WP6122050Chemical Duty 泵，220 V/50 Hz 1
MSCPNPS0096 孔收集板，无色，非无菌 100
MSCPNPP0096 孔 V型底收集板 100
MDCPN2M5096 孔 MultiScreen 深孔接收板，聚丙烯 50
MACL09600MultiScreen 装柱器，100 µL，黑色，铝制，非无菌 1
MACL09680MultiScreen 装柱器，80 µL，黑色，铝制，非无菌 1
MACL09645MultiScreen 装柱器，45 µL，黑色，铝制，非无菌 1
MACL09625MultiScreen 装柱器，25 µL，黑色，铝制，非无菌 1
MSVMHTSHVMultiScreen High Volume Collar, 1 pk 1

装柱器用于装量柱分离的层析培养基。

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Documentation

参考

参考概述应用
Optimization of a simple, automatable extraction method to recover sufficient DNA from low copy number DNA samples for generation of short tandem repeat profiles.
Schiffner LA, Bajda EJ, Prinz M, Sebestyen J, Shaler R, Caragine TA.
Croat Med J. 2005 Aug;46(4):578-86.  2005

A platform for high-throughput molecular characterization of recombinant monoclonal antibodies
Mark J. Bailey, Andrew D. Hooker, Carolyn S. Adams, Shuhong Zhang, David C. James
J. of Chromatography B, 826 (2005) 177-187, 2005  2005

Antibody Purification

问与答

问题回答
The Tech Note "TN053" entitled "Dye Terminator Removal and Sequencing Reaction Clean-up Using Multiscreen 96- Well Filtration Plates" states to add the sequencing reaction to the center of each well (already containing the prepared G-50 resin column). Can I add less than the standard 20ul volumes of sample to each column?Yes. You can add less. When adding volumes less than 10ul you should prewet the resin.
What type of scintillant do you recommend for use in MultiScreen?Although we don't recommend a specific type there are some guidelines. If the reading is done in the MultiScreen plate it important to use a scintilation fluid specific for microtiter plates. The standard scintillation fluid for reading in vials in higher volume and is mixed differently so it shouldn't be used with a microtiter plate.

MicroScint O is for organic and so it would be used with Durapore membrane. In our R&D labs, we have used MicroScint 20 and 40 interchangeably. Supermix (by Wallac) is a microtiter scintillant and has also been used interchangeably with MicroScint 20 and 40.
When I incubate MultiScreen plates, liquid leaks from the bottom of the plate. Is there a way to prevent leakage from the plate?Normally, the Multiscreen filtration plates do not leak when placed on a dry, non absorbent surface during incubation. If you are experiencing leakage, however, be certain that you have asked yourself the following questions:

1. Do I have a high surfactant concentration in the sample?
It is important that you not exceed .1% surfactant in each well.

2. Do I have organic solvents in my solution?
Although the Multiscreen plates have a limited tolerance to organic solevents it is important that you have verified that the concentration and type of solvent used in your assay is compatible with both the membrane and plate plastic. Otherwise, a solvent resistant plate may be more appropriate.

3. Is the underdrain coming in contact with an absorbent surface?
If you lay the plate on lab "chucks" it will cause liquid to wick out from the plate.

4. Have I inspected the plate bottom for microdroplets?
Be sure to blot the plate onto absorbent material prior to all incubations.
What is the well depth and maximum volume capacity of a MultiScreen plate?The well depth of a 96 well MultiScreen plate is 1.245 cm. The well depth for a 384 well MultiScreen plate is 1.2 cm. The maximum working volume of a 96 well plate is 300 ul. The maximum working volume for a 384 well plate is 100 ul.
I am using the Multiscreen system and my samples won't flow through the plate. What's wrong?There are a number of reasons why samples might not flow through the MultiScreen system. Be sure that the vacuum line is clear and not clogged. Be certain that your samples do not have a high particulate load. If they do, you may wish to dilute your samples in filtered buffers. Also, if you are working with cells, make sure that you do not have more than 10^6 cells in each well. If you are over this amount, dilute the cell suspension ot try working with a more open membrane ( larger pore size). Be certain that you do not have the lid on the Multiscreen system. this should be removed prior to filtering in the vacuum manifold. Finally , be sure that if you are not using a whole plate that you have sealed the empty wells with plate sealing tape.

In addition, ensure that the MultiScreen plate is forming a secure seal with the top gasket of the MultiScreen manifold. This can be checked by trying to lift the plate off the manifold with the vacuum on. If the seal is not secure press the four corners of the plate with the vacuum on to seal the plate.
I am using the Multiscreen system and the Multiple Punch assembly for liquid scintillation counting and I am not getting any counts. What might be causing this?Low or inconsistent counts most commonly results from the lack of release of the "labeled" material from the membrane. This is especially an issue with tritium. Millipore has developed a procedure for the release of radioactive label from membrane by adding 0.5 ml of dilute (0.42%) sodium hypochlorite to the scintillation vial containing the "punched" membrane and agitating for thirty minutes prior to the addition of the scintillation cocktail. Please request Technical Brief TB038 for additional details on this procedure.
I can't get any flow through the MultiScreen NA (lysate clearing plate) when performing the plasmid prep protocol. What's wrong?Clogging of the plate is usually due to inappropriate culture medium resulting in overgrowth or lysis. Use 2x LB, not terrific broth or another excessively rich medium.

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Combine the cost savings of bulk media with the convenience of 96-well filtration plates.
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MultiScreen™

种类

Life Science Research > Cell Culture and Systems > Cell Culture Flasks, Plates, & Slides > Multiwell Plates