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95-0100-00 SMCxPRO™ Immunoassay System

The SMCxPRO™ system is the 2nd generation instrument supporting SMC™ technology assays. The SMCxPRO™ technology approach collects signal from analyte fluorochromes free-floating in suspension using a rotating laser, CCD camera optics, and individual photons are counted by Avalanche Photodiode (APD).

95-0100-00
1 system  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number95-0100-00
Trade Name
  • SMC®
DescriptionSMCxPRO™ Immunoassay System
OverviewCombined with our SMC™ immunoassay kits, the newSMCxPRO™ system provides high sensitivity measurements when detecting low levels of protein biomarkers is critical. This affordable, compact system is easy to operate and maintain with streamlined start-up protocol and minimal maintenance requirements.

Network/PC Requirement
• Microsoft Windows® 7/10 Operating Systems
• A static IP address and an FTP server
• xPRO Operating and Analysis Software included (32- or 64-bit compliant)

Read Plate Format
• 384-well plate

Assay Format
• Bead-based assays
• Plate-based assays

Instrument Specifications
• Instrument dimensions: 406.4 mm H x 355.6 mm W x 444.5 mm D (16"H x 14"W x 17.5"D)
• Weight: 22.7 kg (50 lbs)
• US: 115 VAC, 50-60 Hz (Operating range 90-125 V)
• International: 230 VAC, 50-60 Hz (Operating range 180-250 V)

Min. Instrument Performance Specifications
Metric Specification
Slope >1.2 response/fM (determined from calibrator set: 0-300 fM of 150 kD antibody labeled with fluorophore)
Background <3 response/fM (determined from calibrator set: 0-300 fM of 150 kD antibody labeled with fluorophore)
Limit of Detection (LoD) <1 fM
Precision <10% CV (response measurements from 30fM calibrator, n=20)
Dynamic Range >4 logs

Installation and Training
• Set up of unit on bench and complete calibration of unit
• Run of ASSIST program to verify instrument performance
• Optional IQ/OQ and PQ Services available

Three Levels of Onsite Service Support for SMCxPRO™ Platform
• Total Service: Unlimited visits including labor, travel, expenses and all parts for any service for one year
• Advanced Service: Labor, travel and expenses for one preventative maintenance and one floating visit for one year
• Essential Service: Labor for one preventative maintenance visit for one year

Advanced Customer Training
Take advantage of ultrasensitive digital (SMC™) technology while developing your own application-specific immunoassays in either bead- or plate-based assay format. We offer hands-on training at your facility or at one of our global training centers. This training will provide the tools you need to develop your own immunoassays for use on the SMCxPRO™ instrument. Ask your sales specialist for more information.
Background InformationSingle Molecule Counting (SMC™) Technology: Reduced background + increased signal
SMC™ technology provides maximum immunoassay performance while following a workflow similar to traditional ELISA technology. By combining a unique assay elution step and robust digital counting, SMC™ technology achieves improved signal to noise ratios over traditional immunoassay technologies. The SMC™ technology thus provides enhanced quantification at both low and high levels of expression on one complete system.

SMC™ Assay Workflow
During the capture and detection steps, specific antibodies translate each biomarker into a signal. During the modified elution step, fluorescent dye-labeled detection antibodies are dissociated from the immunocomplex. The SMCxPRO™ system is the second generation instrument supporting SMC™ technology assays. The SMCxPRO™ technology approach collects signal from analyte fluorochromes free-floating in suspension using a rotating laser, CCD camera optics, and individual photons are counted by Avalanche Photodiode (APD).

Digital counting improves sensitivity and dynamic range
The SMCxPRO™ instrument captures the sum of all digital events counted above a background threshold. The SMCxPRO™ instrument digital event algorithm computes digital events across a spectrum of time series for a single standard curve, thus improving assay sensitivity
and extending the dynamic measuring range beyond what can be achieved with traditional technologies.
References
Product Information
Detection methodFluorescent
Applications
ApplicationThe SMCxPRO™ system is the 2nd generation instrument supporting SMC™ technology assays. The SMCxPRO™ technology approach collects signal from analyte fluorochromes free-floating in suspension using a rotating laser, CCD camera optics, and individual photons are counted by Avalanche Photodiode (APD).
Key Applications
  • Single Molecule Counting (SMC®)
  • Protein Quantitation
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 system
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
95-0100-00 04054839367823

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