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03-0180-00 SMC™ Human VEGF-A High Sensitivity Immunoassay Kit

The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human VEGF-A High Sensitivity Immunoassay Kit unparalleled performance in the quantification of VEGF-A in human serum and plasma.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Single Molecule Counting (SMC®) Research Category: Inflammation & Immunology Research Sub-Category: Inflammatory Disease, Immune Response, Autoimmune disease
03-0180-00
96 well assay  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityKey Applications
NhP, Fe, CaSingle Molecule Counting (SMC®)
Description
Catalogue Number03-0180-00
Trade Name
  • SMC®
DescriptionSMC™ Human VEGF-A High Sensitivity Immunoassay Kit
OverviewThe SMC™ Human VEGF-A High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
Background InformationVascular Endothelial Cell Growth Factor A (VEGF-A), one of five members of the VEGF family (VEGF-A, VEGF-B, VEGF-C, VEGF-D and Placenta Growth Factor), is a signaling protein critical in angiogenesis and vasculogenesis, predominantly acting on vascular endothelial cells. It has an important role in embryonic development and in wound healing in adults but overexpression in disease can lead to conditions such as vascular disease in the retina, tumor development and growth due to enhanced blood supply while a decrease in VEGF in pulmonary arteries is associated with pulmonary emphysema.
References
Product Information
Components
  • Assay Buffer
  • VEGF Coated Beads
  • Standard Diluent
  • VEGF Detection Antibody
  • VEGF Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B
Precision, %
  • Intra-assay CV% average (n=20): <15%
  • Inter-assay CV% average (n=5): <20%
Applications
ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human VEGF-A High Sensitivity Immunoassay Kit unparalleled performance in the quantification of VEGF-A in human serum and plasma.
Key Applications
  • Single Molecule Counting (SMC®)
Application NotesThis new streamline kit has been improved with the following features including new including new improvements over the legacy VEGF kit, #03-0068-00:
• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Removal of a plate transfer step
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO™ and Erenna®
Biological Information
Species Reactivity
  • Non-human Primate
  • Feline
  • Canine
Species Reactivity NoteAlthough VEGF was detectable in non-human primate, feline, and canine plasma, the assay has not been validated for these species.
Cross ReactivityThe SMC™ Human VEGF-A High Sensitivity Immunoasay Kit was not found to be cross-reactive with the following:
rhVEGF-B
rhVEGF-C
rhVEGF-D
Accuracy
  • Minimum of 5 serum samples spiked with average recovery of 103% with a range of 95-110%
  • Minimum of 5 plasma samples spiked with average recovery of 93% with a range of 80-107%
Physicochemical Information
Sensitivity
  • Lower Limit of Quantification: 0.20 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
03-0180-00 04061841005094