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03-0195-00 SMC™ Human IL-2 High Sensitivity Immunoassay Kit

The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-2 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-2 in human serum and plasma.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Single Molecule Counting (SMC®) Research Category: Inflammation & Immunology Research Sub-Category: Inflammatory Disease, Immune Response, Autoimmune disease, Oncology
03-0195-00
96 well assay  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Key Applications
Single Molecule Counting (SMC®)
Description
Catalogue Number03-0195-00
Trade Name
  • SMC®
DescriptionSMC™ Human IL-2 High Sensitivity Immunoassay Kit
OverviewThe SMC™ Human IL-2 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
Background InformationHuman Interleukin-2 (IL-2) is a signaling cytokine that is primarily produced by activated CD4+ and CD8+ T lymphocytes, and functions in activation and regulation of antigen-specific adaptive immune response. Differentiation of activated T cells into memory T cells, and the differentiation of immature T cells into regulatory T cells are two of the many essential functions that are promoted by IL-2. Partly due to its crucial function in antigen-mediated immunity, IL-2 has been a molecule of interest in translational disease research for decades, with oncology and autoimmunity being two research areas where IL-2 has been particularly relevant as potential therapies. The SMC™ Human IL-2 High Sensitivity Immunoassay Kit harnesses the power of Single Molecule Counting technology to enable researchers to thoroughly track IL-2 in pharmacokinetic studies, as well as evaluate the modulation of this cytokine as a disease biomarker in response to targeted treatment in clinical research, and quantitate subtle differences in expression of this analyte across study conditions.
References
Product Information
Components
  • Assay Buffer
  • IL-2 Coated Beads
  • Standard Diluent
  • IL-2 Detection Antibody
  • IL-2 Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B
HS Code3002 15 90
Precision, %
  • The assay variations of SMC™ Human IL-2 Immunoassay kit were studied
    using five normal plasma samples run in triplicate by 3 different operators on 3 different days.
  • Mean intra-assay variation was 5.5%.
  • Mean inter-assay variation was 10%.
Applications
ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-2 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-2 in human serum and plasma.
Key Applications
  • Single Molecule Counting (SMC®)
Application NotesThis new streamline kit has the following features including:
• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO™ and Erenna®
Biological Information
Species Reactivity NoteSpecificity to the following species samples were tested with the follow results:
Mouse – not cross-reactive
Rat – not cross-reactive
Feline – 25%
Canine – 75%
Non Human Primate – 50%
Cross ReactivityN/A
Accuracy
  • Minimum of 5 serum samples spiked with average recovery of 91% with a range of 79-99%
  • Minimum of 5 plasma samples spiked with average recovery of 83% with a range of 78-87%
Physicochemical Information
Sensitivity
  • Lower Limit of Quantification: 0.09 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
03-0195-00 04065265645614