Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

554715 Retro-2 - CAS 304455-60-5 - Calbiochem

A cell-permeable tricyclic imine compound that inhibits the endosome-to-Golgi retrograde transport, but not cellular uptake, of CtxB, StxB without affecting cellular uptake/trafficking of Tf or EGF.

Retro-2 - CAS 304455-60-5 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

同义词: Cholera Toxin Retrograde Transport Blocker, Ricin Retrograde Transport Blocker, SLT Retrograde Transport Blocker, 2-(((5-Methyl-2-thienyl)methylene)amino)-N-phenylbenzamide

554715
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

CAS #Empirical Formula
304455-60-5C₁₉H₁₆N₂OS

Products

产品目录编号包装 数量 / 包装
554715-25MGCN 玻璃瓶 25 mg
Description
OverviewA cell-permeable tricyclic imine compound that inhibits the endosome-to-Golgi retrograde transport, but not cellular uptake, of CtxB (Cholera toxin B chain), StxB (Shiga-like toxin B chain), and the plant toxin ricin from castor seed in HeLa cells (20 µM), without affecting cellular uptake and trafficking of Tf (Transferrin), EGF, and vesicular stomatitis virus glycoprotein VSVGts045. Retro-2 is demonstrated to selectively affect the cellular distribution of SNARE proteins Synt5 and Synt6, but not 24 other Golgi and endocytic pathway proteins. Retro-2 pretreatment is shown to effectively protect against ricin-, Stx1-, and Stx2-induced cytotoxicity in HeLa cultures in vitro (Fold of protection against 4 h toxin incubation = 2.7, 22, and 65, respectively, with 0.5 h 20 µM Retro-2 pretreatment), as well as ricin-induced (2 µg/kg by nasal spray) fatality in mice in vivo (0 mortality with one 200 mg/kg i.p. dose 1h prior to toxin exposure).
Catalogue Number554715
Brand Family Calbiochem®
SynonymsCholera Toxin Retrograde Transport Blocker, Ricin Retrograde Transport Blocker, SLT Retrograde Transport Blocker, 2-(((5-Methyl-2-thienyl)methylene)amino)-N-phenylbenzamide
References
ReferencesStechmann, B., et al. 2010. Cell 141, 231.
Product Information
CAS number304455-60-5
FormTan powder
Hill FormulaC₁₉H₁₆N₂OS
Chemical formulaC₁₉H₁₆N₂OS
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
554715-25MGCN 04055977193084

Documentation

Retro-2 - CAS 304455-60-5 - Calbiochem MSDS

职位

物料安全数据表 (MSDS) 

Retro-2 - CAS 304455-60-5 - Calbiochem 分析证书

标题批号
554715

参考

参考信息概述
Stechmann, B., et al. 2010. Cell 141, 231.
数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-October-2011 RFH
SynonymsCholera Toxin Retrograde Transport Blocker, Ricin Retrograde Transport Blocker, SLT Retrograde Transport Blocker, 2-(((5-Methyl-2-thienyl)methylene)amino)-N-phenylbenzamide
DescriptionA cell-permeable tricyclic imine compound that inhibits the endosome-to-Golgi retrograde transport, but not cellular uptake, of CtxB (Cholera toxin B chain), StxB (Shiga-like toxin B chain), and the plant toxin ricin from castor seed in HeLa cells (20 µM), without affecting cellular uptake and trafficking of Tf (Transferrin), EGF, and vesicular stomatitis virus glycoprotein VSVGts045. Retro-2 is demonstrated to selectively affect the cellular distribution of SNARE proteins Synt5 and Synt6, but not 24 other Golgi and endocytic pathway proteins. Retro-2 pretreatment is shown to effectively protect against ricin-, Stx1-, and Stx2-induced cytotoxicity in HeLa cultures in vitro (Fold of protection against 4 h toxin incubation = 2.7, 22, and 65, respectively, with 0.5 h 20 µM Retro-2 pretreatment), as well as ricin-induced (2 µg/kg by nasal spray) fatality in mice in vivo (0 mortality with one 200 mg/kg i.p. dose 1h prior to toxin exposure).
FormTan powder
Intert gas (Yes/No) Packaged under inert gas
CAS number304455-60-5
Chemical formulaC₁₉H₁₆N₂OS
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (50 mg/ml). It is recommended to prepare a stock solution for 3 month use each time and store the unreconstituted material in solid form, protected from moisture and preferably under inert gas, for best long-term stability during storage.
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesStechmann, B., et al. 2010. Cell 141, 231.