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HTS040RTA Ready-to-Assay™ NOP1 Opioid Receptor Frozen Cells

Human NOP / ORL1 / OP4 GPCR frozen cells for Calcium Flux FLIPR Assays.

Host Cell: Chem-1 Entrez Gene Number: NM_000913
HTS040RTA
1 kit  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

SpeciesHost CellsProtein Target
Human Chem-1NOP / ORL1 / OP4
Description
Catalogue NumberHTS040RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ NOP1 Opioid Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

The NOP1 receptor (also known as ORL1) is related to the opioid receptor family of GPCRs but does not bind to classical opioids. An endogenous ligand for NOP1 has been characterized and termed orphanin FQ or nociceptin (OFQ/N), which in turn does not bind to other members of the opioid receptor family. NOP1 is expressed widely in the CNS, and binding of OFQ/N to NOP1 appears to function in nociception, locomotor activity, anxiety, reward, memory and tolerance to classical opioids (Mogil and Pasternak, 2001).Millipore’s cloned human NOP1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant NOP1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at NOP1.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ100
Applications
ApplicationHuman NOP / ORL1 / OP4 GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsChem-1
Protein TargetNOP / ORL1 / OP4
Target Sub-FamilyOpioid
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
HTS040RTA 04053252518720

Documentation

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Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells