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03-110 Sigma-AldrichRIPAb+ Ago2 - RIP Validated Antibody and Primer Set

This RIPAb+ Ago2 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考

Key Applications: RNA Binding Protein Immunoprecipitation (RIP), Immunoprecipitation, Western Blotting Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins, RNA Binding Protein (RBP)

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications
H, B	RIP, IP, WB

Description
Catalogue Number	03-110
Brand Family	Upstate
Trade Name	RIPAb+ Upstate
Description	RIPAb+ Ago2 - RIP Validated Antibody and Primer Set
Overview	RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
Alternate Names	Argonaute-2 Eukaryotic translation initiation factor 2C Slicer protein Piwi domain protein
Background Information	Ago2 (argonaute-2), also known as eukaryotic translation initiation factor 2C (EIF2C2), is an essential component for siRNA-directed RNA interference (RNAi) response. Ago2 is an endonuclease required for the unwinding of siRNA duplex and assembly of siRNA into RNA-induced silencing complexes (RISC). Ago2 interacts with DICER1 through its Piwi domain. This Piwi domain is thought to provide RNA cleavage activity via a mechanism similar to RNase H. Ago2 activity is necessary for embryonic development as well as RNA-mediated gene silencing (RNAi).

References

Product Information
Format	Ascites
Control	Includes negative control mouse IgG antibody and control primers specific for human FOS.
Presentation	Anti-Ago2 (Mouse Monoclonal IgG1κ). One vial containing 50 µL of ascites with 0.05% sodium azide. Store at -20°C. Normal Mouse IgG. One vial containing 125 μg of purified mouse IgG in 125 µL of stoage buffer containing 0.1% sodium azide. Store at -20°C. RIP Primers, FOS. One vial containing 75 μL of 5 μM of each primer specific for human FOS gene. Store at -20°C. FOR: GAG AGC TGG TAG TTA GTA GCA TGT TGA REV: AAT TCC AAT AAT GAA CCC AAT AGA TTA GTT A
Quality Level	MQ100

Applications
Application	This RIPAb+ Ago2 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Key Applications	RNA Binding Protein Immunoprecipitation (RIP) Immunoprecipitation Western Blotting
Application Notes	RNA Binding Protein Immunoprecipitation (microRNA): RIP Lysate prepared from HeLa cells (2 X 10⁷ cell equivalents per IP) were subjected to immunoprecipitation using 5 µL of either a normal mouse IgG or 5 µL of Anti-Ago2 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of Ago2-associated microRNA was verified by TaqMan® microRNA assay, hsa-miR-23a. (Please see figures). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details. Immunoprecipitation from RIP lysate: Representative lot data. RIP lysate from HeLa cells (2 X 10⁶ cell equivalents per IP) was subjected to immunoprecipitation using 0.5 µL of either a normal mouse IgG or Anti-Ago2 antibody. Precipitated proteins (lane 1: mouse IgG, lane 2: anti-Ago2) and HeLa whole cell lysate (lane 3) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Ago2 antibody (1:5,000 dilution). Proteins were visualized using goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection. (Please see figures).

Biological Information
Immunogen	KLH-conjugated, synthetic peptide containing the sequence YSGAGPALAPPAPPPPIQG.
Host	Mouse
Specificity	Recognizes Ago2.
Isotype	IgG1κ
Species Reactivity	Human Bovine
Species Reactivity Note	Human. Predicted to react with bovine Ago2 based on sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001158095.1
Entrez Gene Summary	This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene Symbol	Ago2 eIF-2C 2 eIF2C 2 PPD
UniProt Number	Q9UKV8
UniProt Summary	FUNCTION: SwissProt: P45973 # Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Can interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. SIZE: 191 amino acids; 22225 Da SUBUNIT: Interacts with SUV420H1 and SUV420H2 (By similarity). Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys-9'. Interacts with MIS12 and C20orf127. SUBCELLULAR LOCATION: Nucleus. Centromere. Note=Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. PTM: Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper- phosphorylated during mitosis. SIMILARITY: SwissProt: P45973 ## Contains 2 chromo domains.
Molecular Weight	100 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	RNA Binding Protein Immunoprecipitation: RIP Lysate prepared from HeLa cells (2 X 10⁷ cell equivalents per IP) were subjected to immunoprecipitation using 5 µL of either a normal mouse IgG or 5 µL of Anti-Ago2 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of Ago2-associated RNA was verified by qPCR using RIP Primers FOS (Please see figures). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (2 X 10⁷ cell equivalents per IP) were subjected to immunoprecipitation using 5 µL of either a normal mouse IgG or 5 µL of Anti-Ago2 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of Ago2-associated RNA was verified by qPCR using RIP Primers FOS (Please see figures).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance

Packaging Information
Material Size	10 assays
Material Package	10 assays per set. Recommended use: ~5 μL of antibody per RIP (dependent upon biological context).

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
03-110	04053252679209

Documentation

RIPAb+ Ago2 - RIP Validated Antibody and Primer Set MSDS

职位
物料安全数据表 (MSDS)

RIPAb+ Ago2 - RIP Validated Antibody and Primer Set 分析证书

标题	批号
RIPAb+ Ago2 - 2327500	2327500
RIPAb+ Ago2 - 2382636	2382636
RIPAb+ Ago2 - 2444084	2444084
RIPAb+ Ago2 - 1987489	1987489
RIPAb+ Ago2 - 2020308	2020308
RIPAb+ Ago2 - 2064825	2064825
RIPAb+ Ago2 - 2121817	2121817
RIPAb+ Ago2 - 2199651	2199651
RIPAb+ Ago2 - 2287223	2287223
RIPAb+ Ago2 - 2488822	2488822

参考

参考概述	公共医疗ID
Argonaute 2 sustains the gene expression program driving human monocytic differentiation of acute myeloid leukemia cells. Iosue, I; Quaranta, R; Masciarelli, S; Fontemaggi, G; Batassa, EM; Bertolami, C; Ottone, T; Divona, M; Salvatori, B; Padula, F; Fatica, A; Lo-Coco, F; Nervi, C; Fazi, F Cell death & disease 4 e926 2013 显示摘要 MicroRNAs are key regulators of many biological processes, including cell differentiation. These small RNAs exert their function assembled in the RNA-induced silencing complexes (RISCs), where members of Argonaute (Ago) family of proteins provide a unique platform for target recognition and gene silencing. Here, by using myeloid cell lines and primary blasts, we show that Ago2 has a key role in human monocytic cell fate determination and in LPS-induced inflammatory response of 1,25-dihydroxyvitamin D3 (D3)-treated myeloid cells. The silencing of Ago2 impairs the D3-dependent miR-17-5p/20a/106a, miR-125b and miR-155 downregulation, the accumulation of their translational targets AML1, VDR and C/EBPβ and monocytic cell differentiation. Moreover, we show that Ago2 is recruited on miR-155 host gene promoter and on the upstream region of an overlapping antisense lncRNA, determining their epigenetic silencing, and miR-155 downregulation. These findings highlight Ago2 as a new factor in myeloid cell fate determination in acute myeloid leukemia cells.	24263100
Characterization of polysaccharide production of haemophilus influenzae Type b and its relationship to bacterial cell growth. Mickie Takagi,Joaquin Cabrera-Crespo,Júlia Baruque-Ramos,Teresa Cristina Zangirolami,Isaias Raw,Martha Massako Tanizaki Applied biochemistry and biotechnology 110 2003 显示摘要 Haemophilus influenzae type b (Hib) causes invasive infections in infants and young children. Vaccines consisting of Hib capsular polysaccharide (polymer of ribosylribitol phosphate [PRP]) conjugated to a protein are effective in the prevention of such infections. The production of capsular polysaccharide type b was studied in three cultivation conditions: single, glucose pulse, and repeated batch. Specific polysaccharide production (Yp/x) was calculated for all experiments, showing the following values: 67 (single-batch cultivation), 71 (glucose pulse), 75 (repeated-batch cultivation, first batch), and 87 mg of PRP/g of dry cell weight (DCW) (repeated-batch cultivation, second batch). Biomass concentration reached approximately 1.8 g of DCW/L, while polysaccharide concentration was about approximately 132 mg/L in the three fermentation runs. Polysaccharide synthesis is associated with cell growth in all studied conditions as established by Kono's analysis and Luedeking-Piret's model.	14515024

参考概述

公共医疗ID

Argonaute 2 sustains the gene expression program driving human monocytic differentiation of acute myeloid leukemia cells.
Iosue, I; Quaranta, R; Masciarelli, S; Fontemaggi, G; Batassa, EM; Bertolami, C; Ottone, T; Divona, M; Salvatori, B; Padula, F; Fatica, A; Lo-Coco, F; Nervi, C; Fazi, F
Cell death & disease 4 e926 2013

显示摘要

24263100

Characterization of polysaccharide production of haemophilus influenzae Type b and its relationship to bacterial cell growth.
Mickie Takagi,Joaquin Cabrera-Crespo,Júlia Baruque-Ramos,Teresa Cristina Zangirolami,Isaias Raw,Martha Massako Tanizaki
Applied biochemistry and biotechnology 110 2003

显示摘要

14515024

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种类

Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Validated Antibodies

Life Science Research > Antibodies and Assays > Primary Antibodies

Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Validated Antibodies

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