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324865 PhosphoDetect™ Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb

This PhosphoDetect™ Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb is validated for use in Immunoblotting for the detection of EGFR (pTyr⁸⁴⁵).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

同义词: Anti-Epidermal Growth Factor Receptor (pTyr⁸⁴⁵)

324865
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概述

Replacement Information

重要规格表

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Products

产品目录编号包装 数量 / 包装
324865-10TCN 塑胶安瓿;塑胶针药瓶 10 t
Description
OverviewRecognizes the ~170 kDa EGFR protein phosphorylated at Tyr845 in EGF-treated A431 cells. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Catalogue Number324865
Brand Family Calbiochem®
SynonymsAnti-Epidermal Growth Factor Receptor (pTyr⁸⁴⁵)
Application Data
Detection of human epidermal growth factor receptor, phosphorylated on Tyr845, by immunoblotting. Samples: Whole cell lysate from A431 cells left unstimulated (lane 1) or stimulated with EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr845) Rabbit pAb (Cat. No. 324865) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
References
ReferencesSieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
Biscardi, J.S., et al. 1999. J. Biol. Chem. 274, 8335.
Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.
Product Information
FormLiquid
FormulationIn Dulbecco's PBS (w/o Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.4.
Positive controlA431 cells stimulated with EGF
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000)
Application CommentsThe immunogen sequence is 100% conserved in mouse and rat and 93% conserved in chicken EGFR, but cross-reactivity has not been tested. When diluted to 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁸⁴⁵ phosphorylation site of human EGFR
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
324865-10TCN 04055977215816

Documentation

PhosphoDetect™ Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb MSDS

职位

物料安全数据表 (MSDS) 

PhosphoDetect™ Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb 分析证书

标题批号
324865

参考

参考信息概述
Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
Biscardi, J.S., et al. 1999. J. Biol. Chem. 274, 8335.
Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.
数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-August-2007 RFH
SynonymsAnti-Epidermal Growth Factor Receptor (pTyr⁸⁴⁵)
ApplicationImmunoblotting (1:1000)
Application Data
Detection of human epidermal growth factor receptor, phosphorylated on Tyr845, by immunoblotting. Samples: Whole cell lysate from A431 cells left unstimulated (lane 1) or stimulated with EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr845) Rabbit pAb (Cat. No. 324865) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic tyrosine phosphorylated peptide, followed by immunoaffinity chromatography. Recognizes the ~185 KDa EGFR protein phosphorylated on Tyr845.
BackgroundEpidermal Growth Factor (EGF) is responsible for a variety of biological effects including mitogenesis and differentiation. The EGF receptor (EGFR) has three domains: the extracellular, transmembrane, and cytoplasmic domain. Upon the binding of EGF to the extracellular domain, dimerization of the receptor occurs and the cytoplasmic domain displays tyrosine kinase activity. This leads to the autophosphorylation of the ~170 kDa glycoprotein on various residues within the cytoplasmic domain as well as the phosphorylation of intracellular substrates. Tyr845 is an autophosphorylation site within EGFR. Phosphorylation of tyrosine 845 is mediated by non-receptor tyrosine kinase c-Src.
HostRabbit
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁸⁴⁵ phosphorylation site of human EGFR
IsotypeIgG
Specieshuman
Positive controlA431 cells stimulated with EGF
FormLiquid
FormulationIn Dulbecco's PBS (w/o Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.4.
Preservative≤0.1% sodium azide
CommentsThe immunogen sequence is 100% conserved in mouse and rat and 93% conserved in chicken EGFR, but cross-reactivity has not been tested. When diluted to 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesSieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
Biscardi, J.S., et al. 1999. J. Biol. Chem. 274, 8335.
Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.