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A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but stable over wide range of pH and temperature. Note: 1KU = 1000 units.
Note: 1 KU = 1000 units.
Catalogue Number
537088
Brand Family
Calbiochem®
References
Product Information
CAS number
9036-06-0
Activity
≥70,000 PUK (proteolytic units)/g dry weight
Unit of Definition
One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
Form
Lyophilized
Applications
Biological Information
Physicochemical Information
Contaminants
Nuclease: not detectable
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECS
UK9595000
Safety Information
R Phrase
R: 20-36/37/38-42/43
Harmful by inhalation. Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation and skin contact.
S Phrase
S: 22-26-36
Do not breathe dust. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Harmful
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Unstable in solution; reconstitute just prior to use.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
17-September-2007 RFH
Description
A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at pH 7.0 but stable over wide ranges of pH and temperature. Has an optimal pH of 7.5.
Form
Lyophilized
CAS number
9036-06-0
RTECS
UK9595000
Contaminants
Nuclease: not detectable
Activity
≥70,000 PUK (proteolytic units)/g dry weight
Unit definition
One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
Solubility
H₂O (10 mg/ml)
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Unstable in solution; reconstitute just prior to use.