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14-525 PKC ζ Protein, active, 10 µg

Active, N-terminal His6-tagged recombinant human PKC zeta, residues 2-end, for use in Kinase Assays.

PKC ζ Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-525
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-525M when ordering the 250 µg size.
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number14-525
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPKC ζ Protein, active, 10 µg
OverviewN-terminal His6-tagged recombinant human PKC zeta, residues 2-end
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal His6-tagged recombinant human PKC zeta, residues 2-end, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryProtein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
Gene Symbol
  • PRKCZ
  • OTTHUMP00000044160
  • nPKC-zeta
  • PKC2
Protein TargetPKCζ
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q05513 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
SIZE: 592 amino acids; 67660 Da
SUBUNIT: Forms a ternary complex with SQSTM1 and KCNAB2. Forms another ternary complex with SQSTM1 and GABRR3. Forms a complex with SQSTM1 and MAP2K5 (By similarity). Interacts with PARD6A, PARD6B, PARD6G and SQSTM1. Part of a complex with PARD3, PARD6A or PARD6B or PARD6G and CDC42 or RAC1. Interacts with CENTA1. Forms a ternary complex composed of SQSTM1 and PAWR. Interacts directly with SQSTM1 (Probable). Interacts with IKBKB.
SUBCELLULAR LOCATION: Cytoplasm. Endosome. Note=In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes.
TISSUE SPECIFICITY: Expressed in brain, and to a lesser extent in lung, kidney and testis.
DOMAIN: SwissProt: Q05513 The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B. & The C1 domain does not bind the diacylglycerol (DAG).
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 OPR domain. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain.
Molecular Weight68.9kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of PKC substrate peptide II
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-525M when ordering the 250 µg size.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
14-525 04053252409387

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Alternative Packsize

产品目录编号 描述
14-525M PKC ζ Protein, active, 250 µg

产品族

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种类

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases