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14-791 PI 3-Kinase (p110α(E542K)/p85α) murine, 10 µg

Complex of N-terminal 6His-tagged recombinant murine p110α full length, containing the mutation E542K & untagged, recombinant, murine p85α full length.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-791
10 µg  Also available in 250μg size --call for pricing and availability and reference catalog number 14-791M when ordering the 250μg size.
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number14-791
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPI 3-Kinase (p110α(E542K)/p85α) murine, 10 µg
OverviewComplex of N-terminal 6His-tagged recombinant murine p110α full length, containing the mutation E542K, and untagged, recombinant, murine p85α full length.
Background InformationThe E542K substitution is a somatic mutation in p110α that has been associated with tumours of the colon and brain. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation. (Kang S. et al., PNAS, (2005);102: 802-807 and Zhao J.J. et al., PNAS, (2005);102:18443-18448).
References
Product Information
Presentation50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 0.2 mM PMSF, 1 mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
Quality LevelMQ300
Applications
ApplicationComplex of N-terminal 6His-tagged recombinant murine p110α full length, containing the mutation E542K & untagged, recombinant, murine p85α full length.
Biological Information
SourceCo-expressed by baculovirus in Sf21 insect cells.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • PIK3CA
  • PI3K
  • PK3CA
Protein TargetPI3Kα/p85α
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyPI3K/PI4K
UniProt Number
Molecular Weightp110α(E542K) MW = 129 kDa, p85α MW = 84 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Packaging Information
Material Size10 µg
Material PackageAlso available in 250μg size --call for pricing and availability and reference catalog number 14-791M when ordering the 250μg size.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
14-791 04053252679087

相关产品和应用

Alternative Packsize

产品目录编号 描述
14-791M PI 3-Kinase (p110α(E542K)/p85α) murine, 250 µg

Accessories

产品目录编号 描述
14-558 PI3 Kinase (p120γ), 20 µg
14-602 PI3 Kinase (p110α/p85α) Protein, active, 20 µg
14-603 PI3 Kinase (p110β/p85α) Protein, active, 20 µg
14-604 PI3 Kinase (p110δ/p85α) Protein, active, 10 µg
14-781 PI3 Kinase (p110α(E545K)/p85α), Murine, 10 µg
14-782 PI 3-Kinase (p110α(E542K)/p85α), Human, 10 µg
14-783 PI3 Kinase (p110α(E545K)/p85α), 10 µg
14-785 PI3 Kinase (p110α/p85α) murine, 10 µg
14-786 PI3 Kinase (p110α/p65α) Murine, 10 µg
14-787 PI3 Kinase (p110α (H1047R)/p85α) Murine, 10 µg
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种类

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases