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14-463M PDGFRβ Protein, active, 250 µg

Active, recombinant human PDGF Receptor β, residues 557-end, containing an N-terminal His6-tag, for use in Kinase Assays.

14-463M
250 µg  Also available in 50 μg size (cat#14-463) and in bulk (cat# 14-463-K).
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number14-463M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPDGFRβ Protein, active, 250 µg
OverviewRecombinant human PDGF Receptor β, residues 557 end, containing an Nterminal His6-tag
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, recombinant human PDGF Receptor β, residues 557-end, containing an N-terminal His6-tag, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.
Gene Symbol
  • PDGFRB
  • PDGFR1
  • CD140b
  • PDGFR
  • JTK12
  • CD140B
  • PDGF-R-beta
Protein TargetPDGFRβ
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P09619 # Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
SIZE: 1106 amino acids; 123968 Da
SUBUNIT: Homodimer, and heterodimer with PDGFRA. Interacts with APS. The autophosphorylated form interacts directly with SHB and with PIK3C2B, maybe indirectly.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P09619 # A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML). & A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia. & A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1. & A chromosomal aberration involving PDGFRB is a cause in many instances of chronic myeloproliferative disorder with eosinophilia (MPE) [MIM:131440]. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein. & A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP- PDGFRB fusion protein.
SIMILARITY: SwissProt: P09619 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
Molecular Weight63.7kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Poly (Glu4-Tyr) substrate peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at 70 °C
Packaging Information
Material Size250 µg
Material PackageAlso available in 50 μg size (cat#14-463) and in bulk (cat# 14-463-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
14-463M 04053252333026

Documentation

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产品目录编号 描述
14-463 PDGFRβ Protein, active, 50 µg

产品族

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种类

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases