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14-544M PAR-1Bα Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant, full-length, human PAR-1B alpha, for use in Kinase Assays.

PAR-1Bα Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-544M
250 µg  Also available in 10 μg size (cat#14-544) and in bulk (cat# 14-544-K).
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number14-544M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPAR-1Bα Protein, active, 250 µg
OverviewN-terminal His6-tagged, recombinant, full-length, human PAR-1B alpha
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal His6-tagged, recombinant, full-length, human PAR-1B alpha, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed by baculovirus in SF21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryEMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. The human EMK1 was shown to be encoded by a single mRNA ubiquitously expressed.
Gene Symbol
  • MARK2
  • MGC99619
  • EMK1
  • PAR-1
Protein TargetPAR-1Ba
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyCAMK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q7KZI7 # Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.
COFACTOR: Magnesium.
SIZE: 788 amino acids; 87911 Da
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein. Note=Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane.
TISSUE SPECIFICITY: High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
SIMILARITY: SwissProt: Q7KZI7 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily. & Contains 1 KA1 (kinase-associated) domain. & Contains 1 protein kinase domain. & Contains 1 UBA domain.
Molecular Weight81.6kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of CHKtide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-544) and in bulk (cat# 14-544-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
14-544M 04053252731235

相关产品和应用

Alternative Packsize

产品目录编号 描述
14-544 PAR-1Bα Protein, active, 10 µg

产品族

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种类

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases