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MAB8140-KC Mouse Anti-Cytomegalovirus Early (1mg) KC

Anti-Cytomegalovirus Antibody, early, clone 5A8.2 detects level of Cytomegalovirus & has been published & validated for use in IF, WB, IH & IP.

Mouse Anti-Cytomegalovirus Early (1mg) KC MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
MAB8140-KC
Bulk packaging. Please inquire.  
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概述

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Description
Catalogue NumberMAB8140-KC
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionMouse Anti-Cytomegalovirus Early (1mg) KC
OverviewThis product is intended for use by IVD manufacturers.
Contact customer service for assistance: oem.reagents@emdmillipore.com
Alternate Names
  • Anti-CMV
References
Product Information
FormatPurified
PresentationPurified ascites. Supplied in PBS buffer, pH 7.4. Contains 0.01% sodium azide and carrier protein. Has been sterile filtered.
Quality LevelMQ300
Applications
ApplicationAnti-Cytomegalovirus Antibody, early, clone 5A8.2 detects level of Cytomegalovirus & has been published & validated for use in IF, WB, IH & IP.
Key Applications
  • Immunofluorescence
  • Western Blotting
  • Immunohistochemistry
  • Immunoprecipitation
Application NotesImmunochemistry.

IFA at 1:3,000-1:6,000 on acetone fixed cells.

Does not work with paraffin embedded tissue sections.

Dilute with buffer pH 7.4-7.6 to desired working volume.

For extensive dilution, protein containing or other stabilizing medium should be used.

Final working dilutions must be determined by end user.
Biological Information
ImmunogenAffinity purified antigen from MRC-5 cells infected with CMV AD169 (ATCC).
Epitopeearly
Clone5A8.2
HostMouse
SpecificityReacts with Immediate Early 2 protein (IE2) and its fragments. Recognizes a can epitope near the carboxy-end that includes an epitope found in IE2 86, IE2 60 and IE2 40. In western blot recognizes proteins at apparent molecular weights of 86, 68-72, 55 and 38 kD. Can detect CMV infection 1 hour post-infection exhibiting nuclear and/or intranuclear inclusion staining with reaches peak intensity at 3 hours.


With CMV the antigens expressed at different times are listed as:

Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.

Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.

Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at +4°C for up to 12 months
Packaging Information
Material SizeBulk packaging. Please inquire.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MAB8140-KC 04053252630125

相关产品和应用

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产品目录编号 描述
MAB8140 Anti-Cytomegalovirus Antibody, early, clone 5A8.2

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies
IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Viruses by Clinical Condition > Congenital Diseases
IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Viruses by Family > Herpesvirus
IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Viruses by Clinical Condition > Transplant Risk
IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Viruses by Clinical Condition > Meningitis