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LSKGEL050 Montage 凝胶回收试剂盒

DNA Gel Extraction

Montage 凝胶回收试剂盒 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
LSKGEL050
50  
Purchase on Sigma-Aldrich

概述

Replacement Information
描述
产品目录编号LSKGEL050
商名
  • Montage
描述Montage 凝胶回收试剂盒
概述Recover 100 to 10,000 bp DNA from agarose gel slices in a single 10-minute spin. The kit consists of a pre-assembled filter device with an agarose gel nebulizer, a microcentrifuge vial, and modified TAE gel extraction buffer (dry).

The device utilizes gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.

Prepared DNA requires no further purification for most applications, including cloning and radioisotopic or fluorescent DNA sequencing. Since agarose gel electrophoresis has high resolving power, the small and large non-specific amplification products that frequently interfere with cloning and sequencing after PCR are completely removed from the product.

  • Minimal hands-on time
  • 10-minute spin
  • Fully-functional DNA


Performance
Typical DNA Recoveries from Agarose Gels

DNA Size (bp) Percent DNA Recoveries

100 78
700 71
1000 77
2027 47
4361 35
9416 32
23130 29

Protocol
1. Excise the band of interest after routine electrophoretic analysis of PCR products or other DNA through an agarose gel.
2. Place the gel slice into the pre-assembled device. Note: Protocol is not compatible with low melting point agarose.
3. Spin at 5,000 x g for 10 minutes to extract gel slurry into filter cup.
4. DNA can be stored in the capped filtrate vial after discarding the sample filter cup and gel nebulizer.
Alternate Names
  • Ultrafree-DA
参考
产品信息
部件
  • 包含 50 个 Ultrafree-DA 装置和 500 mL 的 50x 浓缩
    改良 TAE 缓冲液
色码蓝色
Quality LevelMQ300
应用
应用DNA Gel Extraction
生物信息
理化信息
尺寸
材料信息
装置材料
  • Polypropylene
毒性学信息
GHS安全信息
安全信息
产品使用声明
储存和货运信息
包装信息
数量50
运输信息
补充信息
规格
Global Trade Item Number
产品目录编号 GTIN
LSKGEL050 04053252326288