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FCMAB116P Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70

This Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70 is validated for use in FC, IC for the detection of SSEA-4.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation Gene Symbol: SSEA-4, SSEA4
FCMAB116P
100 tests  
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特卖

概述

特卖

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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, ICCMPhycoerythrinMonoclonal Antibody
Description
Catalogue NumberFCMAB116P
Trade Name
  • Milli-Mark
DescriptionMilli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70
OverviewN-terminal GST-tagged, recombinant human MuRF1 full length, expressed in E.coli. Purified using glutathione sepharose.
Background InformationThe cell-surface markers used routinely to identify undifferentiated human embryonic stem cells (hESCs) were initially characterized as markers for mESCs, mouse embryonic carcinomas (ECs), or human EC cells. Stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 are widely used as cell-surface markers to define both human and mouse ESCs. SSEA-3 and SSEA-4 are expressed on undifferentiated hESCs but not on undifferentiated mESCs.
Product Information
FormatPhycoerythrin
Control
  • Pluripotent human embryonic stem (ES) cells
PresentationPurified mouse monoclonal IgG3 conjugated to PE fluorochrome in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationThis Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70 is validated for use in FC, IC for the detection of SSEA-4.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
Application NotesImmunocytochemical staining of live H9 human ES cells incubated for 30 minutes at 37oC with 1:50 dilution of anti-SSEA-4, clone MC-813-70, PE conjugate (Cat. No. FCMAB116P) monoclonal antibody.
Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Immunocytochemical staining of fixed H9 human ES cells incubated for 2 hours at 2-8oC with 1:50 dilution of anti-SSEA-4, clone MC-813-70, PE conjugated (Cat. No. FCMAB116P) monoclonal antibody.
Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Biological Information
ImmunogenHuman embryonal carcinoma cell line 2102Ep.
CloneMC-813-70
HostMouse
SpecificityThis antibody reacts with the stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES).
No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In ctontrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.
IsotypeIgG3κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • SSEA-4
  • SSEA4
Purification MethodProtein A Purfied
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8oC protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 tests
Global Trade Item Number
产品目录编号 GTIN
FCMAB116P 04053252371301