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17-1010 Magna ChIP-Seq™ ChIP and NGS Library Preparation Kit

Magna ChIP-Seq Chromatin IP & Next Generation Sequencing Library Preparation Kit containing all key reagents for chromatin IP & next generation sequencing library preparation from genomic DNA.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ChIP-seq, Sample Prep Research Category: Epigenetics & Nuclear Function
17-1010
10 library constructions  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Key Applications
ChIP-seq, Sample Prep
Description
Catalogue Number17-1010
Trade Name
  • Magna ChIP
  • Upstate
DescriptionMagna ChIP-Seq™ ChIP and NGS Library Preparation Kit
OverviewChIP-Seq facilitates genome-wide profiling of the interactions of transcription factors, modified histones, and other chromatin associated proteins. By combining chromatin immunoprecipitation with next generation sequencing, laboratories can generate high resolution data on protein-DNA interactions across the genome. For laboratories interested in gaining a deeper understanding of epigenetic mechanisms of gene regulation, ChIP-Seq has become an indispensable tool. The Magna ChIP-Seq Kit simplifies this technique by providing a validated set of reagents, easy-to-follow protocols, controls, and in process quality control guidelines to allow virtually any laboratory to conduct genome-wide analysis of DNA-protein interactions and the profiling of epigenetic marks.

  • Effective ChIP protocol simplifies production and immunoprecipitation of high quality chromatin
  • Reliable ChIP-Seq library construction from as little as 1 ng of purified ChIP DNA
  • Easy-to-follow protocols and in-process quality control guidelines help ensure successful ChIP and library construction in as little as 2 days
  • Magna ChIP A+G bead blend allows the use of antibodies from virtually any species or class
  • Enzymes and buffers provided in convenient master mix formulations streamlines library construction
  • Validate the ChIP procedure using inlcuded positive and negative control antibodies and a control primer set
  • Proven performance through construction and sequencing of genomic DNA libraries on an Illumina Genome Analyzer II
Materials Required but Not DeliveredCells, stimulated or treated as desired
Antibody for chromatin immunoprecipitation
37% Formaldehyde
Taq DNA polymerase and dNTPs
Master Mix for qPCR
DNase and RNase-free sterile H2O
Quant-iT™ dsDNA HS Assay Kit, (Life Technologies catalog #: Q32851)
Oligonucleotide primers and adaptors
Low Elution Volume PCR purification Kit
Low Elution Volume Gel Extraction Kit
Materials for Agarose Gel Analysis
Magna Grip™ Rack 8 well (20-400) or similar magnetic rack.
Instrument capable of quantifying low concentrations of nucleic acids
References
Product Information
Components
  • Magna ChIP A/G (2° to 8°C) -Chromatin IP Reagents
  • Magna ChIP A/G (-20°C) -Chromatin IP Reagents
  • Magna ChIP-Seq Library Construction (-20°C) Reagents
PresentationThree modules containing all key reagents for chromatin immunoprecipitation and next generation sequencing library preparation from genomic DNA.
Quality LevelMQ100
Applications
ApplicationMagna ChIP-Seq Chromatin IP & Next Generation Sequencing Library Preparation Kit containing all key reagents for chromatin IP & next generation sequencing library preparation from genomic DNA.
Key Applications
  • ChIP-seq
  • Sample Prep
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceMagna ChIP-Seq Kit Functional Validation:
ChIP modules are functionally tested in quantitative chromatin immunoprecipitation reactions and each set of library construction reagents is functionally validated together through construction and sequencing of a genomic DNA library on an Illumina Genome Analyzer II.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when used and stored as directed.
Packaging Information
Material Size10 library constructions
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
17-1010 04053252019005

Documentation

Magna ChIP-Seq™ ChIP and NGS Library Preparation Kit MSDS

职位

物料安全数据表 (MSDS) 

小册子

标题
An Introduction to Antibodies and Their Applications
Magna ChIP-Seq™ Kit
Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

技术信息

标题
Magna ChIP™ protein A/G bead blends simplify the ChIP process and reduce background signals
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

数据表

标题
Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

用户指南

标题
Magna ChIP-Seq™ Chromatin Immunoprecipitation and Next Generation Sequencing Library Preparation Kit