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MGAMMAG-300E MILLIPLEX® MAP Mouse IgE Single Plex Magnetic Bead Kit - Isotyping Multiplex Assay

Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulin E (IgE) in mouse serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Isotyping
MGAMMAG-300E
96-well plate  96 Well Plate
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
IgE MMplexLuminex xMAP
Description
Catalogue NumberMGAMMAG-300E
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® MAP Mouse IgE Single Plex Magnetic Bead Kit - Isotyping Multiplex Assay
Background InformationFound only in mammals, Immunoglobulin E (IgE) plays an essential role in allergic reactions, and is predominantly associated with type 1 hypersensitivity. IgE is the least abundant immunoglobulin isotype in normal or topic mammals, present at only 0.05% of IgG serum concentrations. It is capable of triggering robust immune reactions, with up to 10 times the typical level detected in the serum of atopic mammals, although increased levels occur only in approximately 50% of mammals with allergic disorders. IgE binds with high affinity to FcɛRI,
found primarily on mast cells and basophils, priming the cells to recognize specific antigens. Once primed and with repeated exposure to the same antigen, these cells release molecules such as histamines, prostaglandins and
leukotrienes, resulting in an “allergic reaction”.
References
Product Information
Detection methodLuminex xMAP
ConfigurationSingle Plex
Precision, %
  • Intra-Assay < 5%
  • Inter-Assay < 10%
Panel TypeMAGNETIC Immune Response
Quality LevelMQ200
Applications
ApplicationIsotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulin E (IgE) in mouse serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This kit may be used for the analysis of Mouse IgE in Mouse serum, plasma, tissue/cell lysates and tissue culture supernatant samples.
• The assay can be performed in less than 3 hours
• This assay utilizes as little as 2uL of sample (a 1:200 dilution for plasma or serum is recommended; 2uL in 398uL of Assay Buffer). Tissue/ cell culture supernatants are diluted to approximately 1μg/mL in Assay Buffer
Biological Information
SpecificityCross-reactivity between these antibodies and those against other immunoglobulins is non-detectable or negligible.
Species Reactivity
  • Mouse
Analytes Available
  • IgE
Accuracy
  • 82%
Physicochemical Information
Sensitivity
  • 0.16 ng/mL
Standard Curve Range
  • 0.8 – 3500 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 Well Plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MGAMMAG-300E 04055977291155