数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

HCKP2-11K MilliporeMILLIPLEX® Human Immuno-Oncology Checkpoint Protein Panel 2 - Immuno-Oncology Multiplex Assay

The Human Immuno-Oncology Checkpoint Protein Panel 2 bead-based multiplex panel, using the Luminex xMAP technology, enables the simultaneous analysis of 31 immune checkpoint proteins in human serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
技术信息
Protocols

Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Oncology Research Sub-Category: Immune Response, Inflammation, Tumor Markers

Recommended Products

概述

Replacement Information

重要规格表

Analytes Available	Species Reactivity	Key Applications	Detection Methods
CD40 Ligand 4-1BBL/TNFSF9 Arginase-1 B7-H2/ICOSL B7-H3/CD276 5'-NT/CD73 B7-H4/VTCN1 APRIL/TNFSF13 B7-H5/VISTA CD25/IL-2Rα B7-H6 CD137/4-1BB Granzyme B CD226/DNAM-1 CD30/TNFRSF8 E-Cadherin FGL1/Hepassocin Galectin-1 (GAL1) Galectin-3 Granulysin IDO1 MICA MICB Nectin-2 BAFF/Blys Nectin-4 OX40/CD134 PVR/CD155 Siglec-7 Siglec-9 Perforin	H	Mplex	Luminex xMAP

Description
Catalogue Number	HCKP2-11K
Trade Name	MILLIPLEX
Description	MILLIPLEX® Human Immuno-Oncology Checkpoint Protein Panel 2 - Immuno-Oncology Multiplex Assay
Overview	EMD Millipore’s MILLIPLEX® Human Immuno-Oncology Checkpoint Protein Panel 2 is a 31-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue culture samples: 4-1BBL/TNFSF9, 5’-NT/CD73 (5'-nucleotidase), APRIL (TNFSF13), Arginase-1, B7-H2/ICOSL (Inducible T cell costimulator ligand), B7-H3/CD276, B7-H4/VTCN1 (V-set domain-containing T-cell activation inhibitor 1), B7-H5/VISTA (V-set immunoregulatory receptor), B7-H6 (Natural killer cell cytotoxicity receptor 3 ligand), BAFF/BLyS (TNFSF13B), CD25/IL-2Rα (Interleukin-2 receptor alpha), CD30/TNFRSF8, CD40L (CD40 ligand), CD137/4-1BB (TNFRSF9), CD226/DNAM-1 (DNAX accessory molecule 1), E-Cadherin, FGL1/Hepassocin (Fibrinogen-like protein 1), Galectin-1 (Gal-1), Galectin-3 (Gal-3), Granulysin, Granzyme B, IDO1 (Indoleamine 2,3-dioxygenase 1), MICA (MHC class I polypeptide-related sequence A), MICB (MHC class I polypeptide-related sequence B), Nectin-2 (PVRL2, CD112), Nectin-4 (PVRL4), OX40/CD134 (TNFRSF4), Perforin, PVR/CD155 (Poliovirus receptor cell adhesion molecule), Siglec-7 (Sialic acid-binding Ig-like lectin 7, CD328), Siglec-9 (Sialic acid-binding Ig-like lectin 9, CD329).
Background Information	Immune checkpoint proteins are regulators of immune activation and play a key role in maintaining immune homeostasis, preventing autoimmunity, and are involved in cancer immune escape. These immune system molecules demonstrate the ability to act both as agonists (co-stimulatory checkpoint molecules) or antagonists (co-inhibitory checkpoint molecules) of immune response. Immune checkpoint inhibitor drugs targeting inhibitory immune checkpoint proteins, such as CTLA-4 and PD-L1, have demonstrated efficacy in cancer immunotherapy by improving the antitumor immune response and saving lives in the treatment of many malignancies. Immune checkpoint proteins may express on immune cells and/or tumor cells as activating receptors or inhibitory receptors. Many immune checkpoint proteins are also expressed as soluble forms in circulation and in the tumor and tumor microenvironment and are putative biomarkers for immunotherapy in various diseases including cancer.

References

Product Information
Detection method	Luminex xMAP
Configuration	Design your multiplex kit by choosing available analytes within this panel.
Precision, %	Inter-assay: <15 Intra-assay: <10
Panel Type	MAGNETIC Circulating Cancer

Applications
Application	The Human Immuno-Oncology Checkpoint Protein Panel 2 bead-based multiplex panel, using the Luminex xMAP technology, enables the simultaneous analysis of 31 immune checkpoint proteins in human serum, plasma and cell culture samples.
Key Applications	Multiplexing
Application Notes	This is an overnight incubation assay (16-18 hours at 2-8°C). A maximum of 25 μL per well 1:2 diluted serum or plasma can be used. Tissue culture or other media may be run neat or appropriately diluted in control medium. Note: Best results using cell/tissue lysate samples may require diluting in assay buffer while testing similarly diluted lysis buffer for matrix effects.

Biological Information
Specificity	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity	Human
Analytes Available	CD40 Ligand 4-1BBL/TNFSF9 Arginase-1 B7-H2/ICOSL B7-H3/CD276 5'-NT/CD73 B7-H4/VTCN1 APRIL/TNFSF13 B7-H5/VISTA CD25/IL-2Rα B7-H6 CD137/4-1BB Granzyme B CD226/DNAM-1 CD30/TNFRSF8 E-Cadherin FGL1/Hepassocin Galectin-1 (GAL1) Galectin-3 Granulysin IDO1 MICA MICB Nectin-2 BAFF/Blys Nectin-4 OX40/CD134 PVR/CD155 Siglec-7 Siglec-9 Perforin
Cross Reactivity	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy	CD40L: 87% 4-1BBL/TNFSF9: 89% Arginase-1: 87% B7-H2/ICOSL: 87% B7-H3/CD276: 73% 5’-NT/CD73: 89% B7-H4/VTCN1: 93% APRIL: 84% B7-H5/VISTA: 91% CD25/IL-2Rα: 86% B7-H6: 88% CD137/4-1BB: 88% Granzyme B: 90% CD226/DNAM-1: 82% CD30/TNFRSF8: 94% E-Cadherin: 90% FGL1/Hepassocin: 87% Galectin-1: 89% Galectin-3: 90% Granulysin: 85% IDO1: 89% MICA: 94% MICB: 92% Nectin-2: 83% BAFF/BLyS: 91% Nectin-4: 91% OX40/CD134: 92% PVR/CD155: 89% Siglec-7: 91% Siglec-9: 90% Perforin: 88%

Physicochemical Information
Sensitivity	Refer to kit protocol for sensitives for individual analytes.
Standard Curve Range	CD40L: 3.66 – 15,000 pg/mL 4-1BBL/TNFSF9: 48.8 – 200,000 Arginase-1: 29.3 – 120,000 pg/mL B7-H2/ICOSL: 12.2 – 50,000 pg/mL B7-H3/CD276: 195 – 800,000 pg/mL 5’-NT/CD73: 24.4 – 100,000 pg/mL B7-H4/VTCN1: 391 – 1,600,000 pg/mL APRIL: 2.44 – 10,000 pg/mL B7-H5/VISTA: 18.3 – 75,000 pg/mL CD25/IL-2Rα: 24.4 – 100,000 pg/mL B7-H6: 6.10 – 25,000 pg/mL CD137/4-1BB: 2.44 – 10,000 pg/mL Granzyme B: 1.22 – 5,000 pg/mL CD226/DNAM-1: 195 – 800,000 pg/mL CD30/TNFRSF8: 24.4 – 100,000 pg/mL E-Cadherin: 366 – 1,500,000 pg/mL FGL1/Hepassocin: 48.8 – 200,000 pg/mL Galectin-1: 61.0 – 250,000 pg/mL Galectin-3: 48.8 – 200,000 pg/mL Granulysin: 3.66 – 15,000 pg/mL IDO1: 36.6 – 150,000 pg/mL MICA: 12.2 – 50,000 pg/mL MICB: 104 – 425,000 pg/mL Nectin-2: 24.4 – 100,000 pg/mL BAFF/BLyS: 12.2 – 50,000 pg/mL Nectin-4: 24.4 – 100,000 pg/mL OX40/CD134: 12.2 – 50,000 pg/mL PVR/CD155: 85.4 – 350,000 pg/mL Siglec-7: 3.66 – 15,000 pg/mL Siglec-9: 2.44 – 10,000 pg/mL Perforin: 24.4 – 100,000 pg/mL

Physicochemical Information

Sensitivity

Refer to kit protocol for sensitives for individual analytes.

Standard Curve Range

CD40L: 3.66 – 15,000 pg/mL
4-1BBL/TNFSF9: 48.8 – 200,000
Arginase-1: 29.3 – 120,000 pg/mL
B7-H2/ICOSL: 12.2 – 50,000 pg/mL
B7-H3/CD276: 195 – 800,000 pg/mL
5’-NT/CD73: 24.4 – 100,000 pg/mL
B7-H4/VTCN1: 391 – 1,600,000 pg/mL
APRIL: 2.44 – 10,000 pg/mL
B7-H5/VISTA: 18.3 – 75,000 pg/mL
CD25/IL-2Rα: 24.4 – 100,000 pg/mL
B7-H6: 6.10 – 25,000 pg/mL
CD137/4-1BB: 2.44 – 10,000 pg/mL
Granzyme B: 1.22 – 5,000 pg/mL
CD226/DNAM-1: 195 – 800,000 pg/mL
CD30/TNFRSF8: 24.4 – 100,000 pg/mL
E-Cadherin: 366 – 1,500,000 pg/mL
FGL1/Hepassocin: 48.8 – 200,000 pg/mL
Galectin-1: 61.0 – 250,000 pg/mL
Galectin-3: 48.8 – 200,000 pg/mL
Granulysin: 3.66 – 15,000 pg/mL
IDO1: 36.6 – 150,000 pg/mL
MICA: 12.2 – 50,000 pg/mL
MICB: 104 – 425,000 pg/mL
Nectin-2: 24.4 – 100,000 pg/mL
BAFF/BLyS: 12.2 – 50,000 pg/mL
Nectin-4: 24.4 – 100,000 pg/mL
OX40/CD134: 12.2 – 50,000 pg/mL
PVR/CD155: 85.4 – 350,000 pg/mL
Siglec-7: 3.66 – 15,000 pg/mL
Siglec-9: 2.44 – 10,000 pg/mL
Perforin: 24.4 – 100,000 pg/mL

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage for kit components is 2 - 8°C.

Packaging Information
Material Size	96-well plate
Material Package	96 well plate

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
HCKP2-11K	04061842309092

Documentation

Protocols

Title
Protocol- HCKP2-11K

MILLIPLEX® Human Immuno-Oncology Checkpoint Protein Panel 2 - Immuno-Oncology Multiplex Assay MSDS

职位
物料安全数据表 (MSDS)

技术信息

标题
Quality Control Range Sheet- HCKP2-104 and HCKP2-204
Quality Control Ranges: MILLIPLEX® MAP Human Immuno-Oncology Checkpoint Protein Panel 2

种类

Life Science Research > Protein Detection and Quantification > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Configurable Kits

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