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48-628MAG MILLIPLEX® 2-Plex Phospho/Total CREB - Cell Signaling Multiplex Assay

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Cell Signaling
48-628MAG
96-well plate  96-well plate
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概述

Replacement Information

重要规格表

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
CREB (Total) CREB (Ser133) H, M, RMplexLuminex xMAP
Description
Catalogue Number48-628MAG
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® 2-Plex Phospho/Total CREB - Cell Signaling Multiplex Assay
OverviewThe MILLIPLEX® 2-Plex Phospho/Total CREB magnetic bead kit has been developed for the simultaneous detection of phosphorylated CREB (Ser133) and total CREB in a single well using the Luminex system. This detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples.
Background InformationCREB (cAMP response element binding protein) is a transcription factor that responds to a diverse array of stimuli including hormones, growth factors and neuronal activity. Transcriptional activation of its target genes are important for diverse cellular functions such as cell survival, growth and differentiation. Although CREB is expressed in various tissues, it plays an important regulatory role in nervous system by promoting neuronal survival, proliferation, differentiation and neurite outgrowth. In addition, CREB signaling is involved in synaptic placidity and long-term memory, and has been implicated in neurodegenerative diseases such as Alzheimer’s and Huntington’s disease.

CREB is phosphorylated on Ser133 by a variety of kinases, such as PKA, CaMK, MSK, and p90RSK. Understanding the regulation and signaling by CREB in cell survival, growth and differentiation will lead to new strategies to treat cancer, neurodegenerative and other diseases.

The MILLIPLEX® 2-Plex Phospho/Total CREB kit contains all the components necessary to measure total CREB and phosphorylated CREB (Ser133).
References
Product Information
Components
  • MILLIPLEX® 2-plex Phospho/Total CREB, Magnetic Beads (20X), 1 tube, 180 uL
  • MILLIPLEX® 2-plex Phospho/Total CREB, Biotin (20X) Detection Antibody, 1 tube, 180 uL
  • MILLIPLEX® Lysis Buffer (1X), 1 bottle, 55 mL
  • MILLIPLEX® Assay Buffer 2 (1X), 1 bottle, 55 mL
  • MILLIPLEX® HeLa Cell Lysate: Phosphatase, 1 vial
  • MILLIPLEX® HeLa Cell Lysate: TNFa/Calyculin A, 1 vial
  • MILLIPLEX® Streptavidin-Phycoerythrin (25X), 1 tube, 150 uL
  • MILLIPLEX® Amplification Buffer (1X), 1 bottle, 3 mL
  • Empty mixing bottles, 3 bottles
  • 96-well Plate, 1 plate and 2 sealers
Detection methodLuminex xMAP
ConfigurationPremixed
Panel TypeCell Signaling
Quality LevelMQ200
Applications
ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
Key Applications
  • Multiplexing
Application Notes• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted, filtered cell lysate per well.
• This kit must be run using the provided Assay Buffer.
• The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 µL/well at 40 to 1,000 µg/mL)
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
  • Mouse
  • Rat
Analytes Available
  • CREB (Total)
  • CREB (Ser133)
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities of individual biomarkers.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
48-628MAG 04055977162585

Documentation

Protocols

Title
Protocol- 48-628MAG

MILLIPLEX® 2-Plex Phospho/Total CREB - Cell Signaling Multiplex Assay MSDS

职位

物料安全数据表 (MSDS) 

小册子

标题
NPI Flyer- Epigenetics and Nuclear Function Feature
New Products - Antibodies, Small Molecule, Inhibitors

技术信息

标题
96 well Template 2 Up
96 well Template Single
Intracellular signal transduction pathways -MILLIPLEX®MAP Cell Signaling Phospho/Total 2-Plex assays
White Paper: Further considerations of antibody validation and usage.